Determination of chidamide in human plasma by high performance liquid chromatography
10.13699/j.cnki.1001-6821.2024.23.017
- VernacularTitle:用高效液相色谱测定人血浆中西达本胺的浓度
- Author:
Jia-xin YU
1
;
Qiang FU
1
;
Xin LIU
1
;
Zhuo SUN
1
;
Ting-ting XU
1
;
Chun-su LIANG
1
;
Bo ZHANG
1
;
Wei ZUO
1
Author Information
1. 中国医学科学院北京协和医学院北京协和医院,药剂科,北京 100730;中国医学科学院北京协和医学院北京协和医院,疑难重症及罕见病国家重点实验室,北京 100730
- Publication Type:Journal Article
- Keywords:
chidamide;
high performance liquid chromatography;
therapeutic drug monitoring
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(23):3449-3452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatographic(HPLC)method for the determination of chidamide in human plasma.Methods The plasma sample was taken as 500 μL,and triamcinolone was used as the internal standard.After liquid-liquid extraction using methyl tert-butyl ether,the supernatant was centrifuged and blown dry under N2,then re-dissolved in 50 μL of water,and then centrifuged again,and then 10 μL of the supernatant was injected into the system.The separation was performed on a Shim-pack CLC-ODS(150 mm×60 mm,5 μm)at 35 ℃ using water-acetonitrile=(74∶26,v/v,adjusted to pH=3 by acetic acid)as mobile phase.The flow rate was 1.0 mL·min-1 and the wavelength was 260 nm.The specificity,standard curve,lower limit of quantitation,precision,recovery and stability of the method were investigated.In addition,the high performance liquid chromatography method described above was applied to determine the plasma drug concentration after oral administration of chidamide in one patient.Results The retention time of chidamide and internal standard was 8.00 and 9.40 min,respectively.The standard curve equation was y=10.28x-0.06(r=0.998).The intra-day and inter-day precision(RSD)of low,medium and high concentration quality control samples(0.02,0.15,0.75 μg·mL-)were 1.00％-4.87％(n=6),and the accuracy was-10.29％-3.37％.The average extraction recovery was 61.74％-69.85％.Conclusion The method was simple,sensitive and accurate,suitable for monitoring the concentrations of chidamide in human plasma.
