Screening for α-glucosidase and tyrosinase inhibitors from Forsythia suspensa leaves by LC-MS technology combined biochemical analysis method
10.13699/j.cnki.1001-6821.2024.23.018
- VernacularTitle:用液质联用法联合生化分析法筛选连翘叶中α-葡萄糖苷酶和酪氨酸酶抑制作用的成分
- Author:
Jing-pu XU
1
;
Xing-long TAO
1
;
Li-ya ZHENG
1
;
De-qiang LI
1
Author Information
1. 河北医科大学第二医院药学部,河北石家庄 050000
- Publication Type:Journal Article
- Keywords:
Forsythia suspensa;
LC-MS technology;
biochemical analysis method;
α-glucosidase;
tyrosinase
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(23):3453-3457
- CountryChina
- Language:Chinese
-
Abstract:
Objective To build a liquid chromatography mass spectrometry technology combined biochemical analysis method for screening α-glucosidase and tyrosinase inhibitors from Forsythia suspense leaves extract.Method Forsythia suspense leaves extract was injected into the high performance liquid chromatography system for the separation and 10 fractions were collected manually according to the peak time of each injection.The bioassays were determined by spectrophotometric method,the high performance liquid chromatography mass spectrometry was used for the characterization.Results A total of 7 fractions were screened from forsythia leaf extract which had inhibitory effects on α-glucosidase and tyrosinase inhibitors,5 compounds were characterized,and the qualitative results showed that fraction 3 was calceolarioside B or plantainoside B,fraction 4 was(+)-epicatechol-4-O-β-D-glucoside,fraction 5 was phillyrin,fraction 6 was the isomeride of phillyrin,and fraction 7 was calceolarioside C.Fraction 3,4 and 5 showed inhibitory effect on both tyrosinase and α-glucosidase,fraction 6 showed inhibitory effect on α-glucosidase only.Conclusion The test results showed that the proposed method was a simple,effective,rapid method for drug discovery in natural products,and it can be extended to screen other bioactive compounds from natural products and other complex samples.
