Research on the intervention of Zhiwei Fuwei pills on rats with precancerous lesions of gastric cancer
10.13699/j.cnki.1001-6821.2024.23.013
- VernacularTitle:制萎扶胃丸对胃癌前病变模型大鼠的干预作用研究
- Author:
Peng-cheng DOU
1
;
Xue-ling YUAN
;
Rui-ping SONG
;
Zhuang-zhuang FENG
;
Liu-yi YAO
;
Xin-yi CHEN
;
Xiao-long WANG
;
Jiao-jiao ZUO
;
Jin SHU
Author Information
1. 甘肃中医药大学中医临床学院,甘肃兰州 730030
- Publication Type:Journal Article
- Keywords:
Zhiwei Fuwei pill;
precancerous lesions of gastric cancer;
hypoxia-inducible factor-1α;
vascular endothelial growth factor;
angiopoietin-2;
vascular endothelial growth factor receptor-2
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(23):3429-3433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the intervention effect and mechanism of Zhiwei Fuwei pills on precancerous lesions of gastric cancer(PLGC)model rats based on hypoxia-inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods PLGC model rats were established by the combination of N-methyl-N'-nitro-N-nitrosoguanidine and ammonia drinking,hunger and satiation disorder,ethanol gavage and ranitidine feeding,and were randomly divided into model group,control group and experimental-H,-M,-L groups,with 8 rats in each group.Another 8 healthy rats were selected as blank group.Experimental-H,-M,-L groups were given 1.67,0.84 and 0.42 g·kg-1 Zhiwei Fuwei pills solution by intragastric administration,respectively;control group was given 2.00 × 10-3g·kg-1 folic acid solution by intragastric administration;blank group and model group were given 0.9％NaCl by intragastric administration,once a day for 4 weeks.The structural changes of gastric microvessels were detected by transmission electron microscopy,and the expression levels of HIF-1α,VEGF,vascular endothelial growth factor receptor-2(VEGFR-2)and Angiopoietin-2(Ang-2)were detected by western blot.Results Compared with the model group,the abnormality of gastric microvascular structure in experimental-H,-M,-L groups was improved to some extent,and the effect of high dose experimental group was the most obvious.The relative expression levels of HIF-1 α protein in experimental-H,-M groups and control group,model group and blank group were 0.43±0.03,0.66±0.04,0.77±0.01,0.80±0.02 and 0.37±0.02;the relative expression levels of VEGF protein were 0.97±0.06,1.21±0.06,1.51±0.07,1.54±0.05 and 0.88±0.02;the relative expression levels of VEGFR-2 protein were 1.03±0.06,1.18±0.04,1.37±0.05,1.45±0.02 and 0.70±0.02;the relative expression levels of Ang-2 protein were 0.51±0.03,0.61±0.02,0.71±0.01,0.78±0.03 and 0.34±0.01,respectively.Compared with the model group,the above indexes in the experimental-H,-M groups were statistically significant(all P＜0.01).Conclusion Zhiwei Fuwei pills may inhibit the abnormal activation of HIF-1α/VEGF signaling pathway,improve hypoxia microenvironment,reduce angiogenesis,and then effectively interfere with the progression of PLGC.
