Research of in vitro pharmacy and in vivo pharmacodynamics of recombinant human thrombin
10.13699/j.cnki.1001-6821.2024.23.011
- VernacularTitle:重组人凝血酶体外药学及体内药效学的研究
- Author:
Wen-feng XU
1
;
Shuo XU
1
;
Shan-shan ZHANG
1
;
Yong-mei KUANG
1
;
Peng-fei JIN
1
Author Information
1. 北京医院、国家老年医学中心、中国医学科学院老年医学研究院药学部、北京市药物临床风险与个体化应用评价重点实验室(北京医院),北京 100730
- Publication Type:Journal Article
- Keywords:
recombinant human thrombin;
protein content;
protein purity;
biological activity;
procoagulant activity
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(23):3420-3423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro pharmaceutical properties(protein content and purity,biological activity)and in vivo pharmacodynamics(procoagulant activity)of recombinant human thrombin.Methods The content of protein in recombinant human thrombin was determined by folin-reagent method.Sodium dodecyl sulphate-polyacrylamide gel electrophoresis was used to determine its purity.Human thrombin activity test was applied to detect its biological activity.Eighteen male New Zealand rabbits were randomly divided according to body mass into blank group(0.9％NaCl solution),experimental-L group(200 U·mL-1 recombinant human thrombin)and experimental-H group(1 000 U·mL-1 recombinant human thrombin),6 rabbits in each group.The procoagulant effects of recombinant human thrombin were investigated in the rabbit liver injury model.Results The protein content of recombinant human thrombin was(1.980±0.024)mg per container,its biological activity was(5764.3±197.7)U per container and its biological specific activity was(2 911.2±99.8)U·mg-1.The target protein(35 kD in non-reductive electrophoresis)has a single band and few miscellaneous proteins were found on the gel.Compared with the blank group,the mean hemostasis time and mean blood loss weight of rabbit liver wound were reduced by 57.0％and 87.7％in the experimental-L group,which in the experimental-H group were reduced by 71.8％and 91.9％.Conclusion Recombinant human thrombin has high protein purity and specific biological activity,and has significant procoagulant activity in vivo.
