Research of paeonol alleviating retinal vascular endothelial cell injury
10.13699/j.cnki.1001-6821.2024.23.010
- VernacularTitle:丹皮酚缓解视网膜血管内皮细胞损伤的研究
- Author:
Yan-xia LIU
1
;
Rui YANG
1
;
Zhi-hui SUN
1
;
Xiao-yan ZHANG
1
;
Yao-jun CHEN
1
;
Lin BAI
1
Author Information
1. 上海市第一人民医院酒泉医院/酒泉市人民医院眼科,甘肃酒泉 735000
- Publication Type:Journal Article
- Keywords:
paeonol;
vascular endothelial cell injury;
diabetic retinopathy;
circular RNA fat mass and obesity associated;
microRNA-128-3p;
thioredoxin-interacting protein
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(23):3414-3419
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of paeonol inhibiting circRNA obesity protein(circFTO)in alleviating retinal vascular endothelial cell injury.Methods Cells were treated with high glucose(HG)to establish a cell injury model.Human retinal microvascular endothelial cells were divided into control group(without any treatment),model group(30 mmol·L-1 HG treatment),si-NC group(30 mmol·L-1 HG+si-NC 50 nmol treatment),si-circFTO group(30 mmol·L-1 HG+si-circFTO 50 nmol treatment),experimental group(30 mmol·L-1 HG+60 μmol·L-1 paeonol treatment),pcDNA3.1-NC group(30 mmol·L-1 HG+60 μmol·L-1 paeonol+pcDNA3.1-NC 2 μg treatment),pcDNA3.1-thioredoxin interacting protein(TXNIP)group(30 mmol·L-1 HG+60 μmol·L-1 paeonol+pcDNA3.1-TXNIP 2 μg treatment).The relative expression level of mRNA was detected by real-time fluorescence quantitative polymerase chain reaction.The expression of TXNIP in each group was detected by immunofluorescence assay.The expression level of miR-128-3p was detected by fluorescence in situ hybridization.The apoptosis of cells was detected by in situ end transferase labeling technique.Results The relative expression levels of circFTO in the control group,model group,si-NC group and si-circFTO group were 1.00±0.15,3.16±0.38,2.97±0.31 and 1.61±0.18,respectively;the relative expression levels of miR-128-3p were 1.00±0.13,0.43±0.05,0.49±0.06 and 0.86±0.09,respectively;the relative expression levels of TXNIP mRNA were 1.00±0.14,1.76±0.21,1.69±0.17 and 1.08±0.12,respectively;the above indexes in the si-circFTO group were statistically different from those in the si-NC group and the control group(all P＜0.001);the relative expression levels of angiopoietin 2(ANG2)mRNA in the model group,experimental group,pcDNA3.1-NC group and pcDNA3.1-TXNIP group were 1.00±0.12,0.68±0.07,0.71±0.08 and 0.97±0.13,respectively;the relative expression levels of tight junction protein 1(ZO-1)mRNA were 1.00±0.15,1.86±0.20,2.18±0.23 and 1.29±0.15,respectively;the proportion of apoptosis were(8.79±1.86)％,(69.52±12.05)％,(66.38±14.61)％and(18.64±3.72)％,respectively.The above indexes of the experimental group were compared with the model group,and the above indexes of the pcDNA3.1-TXNIP group were compared with the pcDNA3.1-NC group;the differences were statistically significant(P＜0.05,P＜0.001).Conclusion Paeonol can alleviate retinal vascular endothelial cell damage,and its mechanism may targetedly up-regulate the level of miR-128-3p and down-regulate the expression of TXNIP by inhibiting the expression of circFTO.
