Expression of ITGBL1 in Colorectal Cancer Tissues and Effects on Biological Function of Colorectal Cancer Cells
10.3969/j.issn.1008-7125.2024.10.007
- VernacularTitle:ITGBL1在结直肠癌组织中的表达及其对结直肠癌细胞生物学功能的影响
- Author:
Xiaoxia ZHU
1
;
Zhijie ZHENG
;
Sihui ZOU
;
Tongguo SHI
;
Rui LI
;
Weichang CHEN
;
Nan GAO
Author Information
1. 苏州大学附属第一医院消化内科(215006)
- Publication Type:Journal Article
- Keywords:
Colorectal Neoplasms;
ITGBL1;
Immunohistochemistry;
Cell Proliferation
- From:
Chinese Journal of Gastroenterology
2024;29(10):616-620
- CountryChina
- Language:Chinese
-
Abstract:
Background:The incidence of colorectal cancer(CRC)remains high in China.ITGBL1,an intergrin-like molecule,may play a significant role in the tumorigenesis and progression of CRC.Aims:To explore the expression of ITGBL1 in CRC tissues,and its clinical significance and effect on the biological function of CRC cells.Methods:Fifty-five paraffin embedding cancerous and paracancerous tissues from CRC patients undergoing surgical treatment were collected.Protein expression of ITGBL1 was detected by immunohistochemistry.The relationship between ITGBL1 expression and the clinicopathological characteristics of the CRC patients was analyzed.Furthermore,the sh-ITGBL1 lentiviral system was used to construct ITGBL1 knockdown stably-transformed HCT116 and RKO cells,then the ITGBL1 protein expression was detected by Western blotting.Effects of ITGBL1 knockdown on the proliferation of CRC cells were analyzed by CCK-8 assay and colony formation assay.Results:ITGBL1 was highly expressed in CRC tissues and cells.Expression of ITGBL1 was positively correlated with the infiltration depth,lymph node metastasis and the clinical stage of CRC(all P＜0.05),whereas no correlations were found between ITGBL expression and the gender,age,tumor size,tumor differentiation,and distant metastasis of CRC patients.Compared with the control group(sh-NC),ITGBL1 expression was significantly decreased in ITGBL1 knockdown(sh-ITGBL1)HCT116 and RKO cells,and the cell proliferation was significantly inhibited(all P＜0.05).Conclusions:Highly expressed ITGBL1 may be associated with the occurrence and development of CRC.ITGBL1 knockdown exerts a significant inhibitory effect on the proliferation of CRC cells.Therefore,ITGBL1 might be a potential biomarker,which is expected to be a new target for diagnosis and treatment of CRC.
