Protective effects of hematoma aspiration combined with hyperbaric oxygen therapy on rat brain tissue with cerebral hemorrhage
10.3760/cma.j.cn311847-20210112-00015
- VernacularTitle:血肿抽吸术联合高压氧治疗对脑出血大鼠脑组织的保护作用
- Author:
Xinguan ZHOU
1
;
Lei WANG
1
;
Dengpeng REN
1
;
Shifeng YANG
1
;
Taihong LI
1
Author Information
1. 运城市中心医院神经外科，运城 044000
- Publication Type:Journal Article
- Keywords:
Hematoma aspiration;
Hyperbaric oxygen;
Cerebral hemorrhage;
JAK2/STAT3 signaling pathway
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2022;29(1):93-98
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the protective effects of hematoma aspiration combined with hyperbaric oxygen（HBO）therapy on the brain tissue of rats after cerebral hemorrhage.Methods:A total of 40 rats were randomly divided into four groups：sham operation group，model group，operation group（hematoma aspiration），and combined treatment group（minimally invasive hematoma aspiration + HBO），with 10 rats in each group. The neurological deficits of rats in each group were scored by Longa scoring method. The water content of the brain tissue of rats in each group was detected by dry/wet weight measurement. The pathological changes in the hippocampus tissue of rats in each group were observed by H&E staining. The number of hippocampal neuronal apoptosis of rats in each group was detected by TUNEL assay. The expression levels of brain derived neurotrophic factor（BDNF）and Aquaporin-4（AQP4）in the hippocampus of rats in each group were detected by qRT-PCR and Western blotting. The protein expressions of Bax，Bcl-2，JAK2，p-JAK2，STAT3，and p-STAT3 in the brain tissue of rats in each group were detected by Western blotting.Results:Compared with the sham operation group，the model group had significantly higher nerve injury function scores and brain tissue water content，and showed brain tissue hemorrhage，cell pyknosis，enlarged intercellular space，increased glial cells，and decreased neurons；the apoptosis level of hippocampal neurons in the model group increased（ P<0.05）；the AQP4，p-JAK2，and p-STAT3 expressions in the model group increased，while the BDNF expression in the model group decreased（ P<0.05）. Compared with the model group，the operation group and the combined treatment group had lower nerve injury function scores and less brain tissue water content，and the brain tissue injury in the two groups was reduced. In both groups，the apoptosis level of hippocampal neurons decreased（ P<0.05）；the AQP4，p-JAK2，and p-STAT3 expressions decreased，while the BDNF expression increased（ P<0.05）. Compared with the operation group，the combined treatment group had significantly lower nerve injury function score and less brain water content，and its cell pyknosis，edema，and other conditions significantly subsided. In the combined treatment group，the apoptosis level of hippocampal neurons decreased（ P<0.05）；the AQP4，p-JAK2，and p-STAT3 expressions decreased，while the BDNF expression increased（ P<0.05）. Conclusion:Hematoma aspiration combined with HBO therapy has a protective effect on rat brain with injury due to cerebral hemorrhage，and the protective mechanism may be related to the inhibition of JAK2/STAT3 signaling pathway．
