Effects of hyperbaric oxygen combined with paclitaxel on cell cycle and ROS level of lung cancer AGS cells
10.3760/cma.j.cn311847-20210716-00209
- VernacularTitle:高压氧联合紫杉醇对人胃癌AGS细胞周期及活性氧水平的影响
- Author:
Yanying XIE
1
;
Ziheng ZHENG
;
Chunjin XU
;
Jianjun ZHAO
Author Information
1. 商丘市第一人民医院消化内科，商丘 476100
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Human gastric cancer AGS cells;
Paclitaxel;
Reactive oxygen species;
Cell cycle
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2022;29(1):89-92,98
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of hyperbaric oxygen（HBO）combined with paclitaxel（PTX）on the proliferation，level of reactive oxygen species（ROS），and cell cycle of human gastric cancer AGS cell line cultured in vitro. Methods:After the completion of in vitro culture，the AGS cells were divided into four groups，including control group，PTX group，HBO group，and HBO+PTX group. Cell Counting Kit-8（CCK-8）assay was used to detect the inhibitory effect of HBO combined with PTX on the proliferation of AGS cells. Flow cytometry was adopted to detect the intracellular ROS level and cell cycle arrest. Western blotting was used to assess the expression levels of cell cycle-related proteins. Results:The survival rate of AGS cells in the HBO+PTX group was significantly lower than those in the control group and the HBO group（ P<0.01），and also lower than that in the PTX group（ P<0.05）. The ROS level of AGS cells in the HBO+PTX group was significantly higher than those in the control group and the HBO group（ P<0.01），and also higher than that in the PTX group（ P<0.05）. PTX blocked the AGS cell cycle in S phase（ P<0.05），and the percentage of cells at S phase in the HBO+PTX group was found slightly higher than that in the PTX group（ P>0.05）. The expression level of Cyclin A1 in the HBO+PTX group was significantly lower than those in the other three groups（ P<0.05 or P<0.01）；the expression level of CDK2 in the HBO+PTX group was lower than those in the control group and the HBO group（ P<0.05），and also slightly lower than that in the PTX group（ P>0.05）. Conclusion:HBO can effectively enhance the inhibitory effect of PTX on the proliferation of AGS cells，increase the intracellular ROS level in AGS cells，and block the cell cycle，thereby inhibiting the differentiation and proliferation of AGS cells.
