Therapeutic effect of baicalin on chronic migraine rats by regulating MIP-1α/CCR1 signaling pathway
10.3969/j.issn.1000-484X.2025.06.033
- VernacularTitle:黄芩苷调节MIP-1α/CCR1信号通路对慢性偏头痛大鼠的治疗作用研究
- Author:
Haifeng GUO
1
;
Xiaoyan ZHANG
1
;
Fei LI
1
;
Chen GAO
1
;
Fengshan SUN
1
Author Information
1. 广安门医院济南医院(济南市中医医院)脑病科,济南 250012
- Publication Type:Journal Article
- Keywords:
Baicalin;
Macrophage inflammatory protein-1α/CC chemokine receptor 1 signaling pathway;
Chronic migraine;
Mechanical pain threshold
- From:
Chinese Journal of Immunology
2025;41(6):1461-1466
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the therapeutic effect and mechanism of Baicalin(BG)on chronic migraine(CM)rats.Methods:CM rat model was established by intermittent subcutaneous injection of nitroglycerin.Rats were randomly grouped into con-trol group,chronic migraine group(CM group),low-dose and high-dose Baicalin groups(BG-L group,BG-H group),Baicalin+MIP-1α recombinant protein group(BG+MIP-1α group)and CCR1 inhibitor ZK811752 group(CCR1-ZK group),with 10 rats in each group.General behavior of rats were observed and scored;von Frey fiber optic pain gauge was applied to measure the mechanical pain threshold of rats;Nissl staining was applied to observe the neuronal structure of ventrolateral periaqueductal gray(vlPAG);ELISA was applied to detect levels of TNF-α and IL-1β;immunohistochemical method was applied to detect expressions of pain related pro-teins c-Fos and CGRP;Western blot was applied to detect expressions of MIP-1α and CCR1 proteins.Results:Compared with Control group,the body mass,mechanical pain threshold,and the number of Nissl bodies in vlPAG area of rats in CM group were obviously reduced,the general behavioral score,levels of TNF-α,IL-1β,and expressions of c-Fos,CGRP,MIP-1α and CCR1 were obviously increased(P<0.05);compared with CM group,the body mass,mechanical pain threshold,the number of Nissl bodies in vlPAG area of rats in BG-L group,BG-H group and CCR1-ZK group were obviously increased,the general behavioral score,levels of TNF-α,IL-1β,and expressions of c-Fos,CGRP,MIP-1α and CCR1 were obviously reduced(P<0.05);compared with BG-H group,the body mass,mechanical pain threshold,and the number of Nissl bodies in vlPAG area of rats in BG+MIP-1α group were obviously reduced,general behavioral score,levels of TNF-α,IL-1β,and expressions of c-Fos,CGRP,MIP-1α and CCR1 were obviously increased(P<0.05).Conclusion:Baicalin has certain therapeutic effect on chronic migraine rats,and its mechanism may be related to inhibiting the activation of MIP-1α/CCR1 signaling pathway.
