Inhibitory effect of a specific synbiotic on colon tumorigenesis in ApcMin/+mice
10.3969/j.issn.1000-4718.2025.07.008
- VernacularTitle:一种特定合生元对ApcMin/+小鼠结肠肿瘤发生的抑制作用
- Author:
Jiao ZHENG
1
;
Huixia WU
;
Yao TONG
;
Hong WEN
;
Rushi LIU
;
Minjing LIAO
Author Information
1. 湖南师范大学医学院,湖南 长沙 410013
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
gut flora;
probiotic;
prebiotic;
synbiotic
- From:
Chinese Journal of Pathophysiology
2025;41(7):1317-1324
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aims to investigate whether a specific synbiotic,consisting of Bifidobacterium longum subspecies BB536 and fructooligosaccharides,can inhibit colon tumorigenesis by modulating gut flora composi-tion.METHODS:Six-week-old male ApcMin/+mice were randomly divided into control group and synbiotic-treated group,with 8 mice in each group.The mice in synbiotic-treated group received daily gavage of 1×109 CFU/kg of Bifidobacterium longum BB536 and 2 g/kg of fructooligosaccharides,while those in control group were gavaged with sterile water for 10 weeks.The number and size of colon tumors were assessed and compared between the 2 groups.Colon tissue sections were evaluated using hematoxylin-eosin staining,Alcian blue staining,and immunohistochemistry assays.The bacterial compo-sition in the colon was analyzed via 16S rDNA sequencing,and differentially expressed genes were examined through tran-scriptome sequencing and RT-qPCR.RESULTS:The synbiotic treatment significantly enhanced bacterial diversity and increased the relative abundance of Bifidobacterium in the colon(P<0.01).Additionally,the number and size of colon tu-mors were both significantly reduced in the synbiotic-treated mice(P<0.01),along with decreased expression of Ki-67(P<0.01).Concurrently,the number of goblet cells and the expression of epithelial tight junction proteins(ZO-1 and occlu-din)were up-regulated(P<0.05).CONCLUSION:Supplementation with this synbiotic effectively modulated gut flora composition and suppressed colon tumorigenesis in ApcMin/+mice.
