Synergistic cytotoxic effect of chrysin and venetoclax on AML cells and its mechanism
10.3969/j.issn.1000-4718.2025.07.006
- VernacularTitle:白杨素协同维奈托克杀伤AML细胞的作用及其机制研究
- Author:
Yan WANG
1
;
Peixiong ZHU
;
Pengyue YANG
;
Xiuli WU
;
Yangqiu LI
;
Xi-bao YU
;
Ling XU
Author Information
1. 暨南大学基础医学与公共卫生学院血液学研究所,再生医学教育部重点实验室,广东 广州 510632
- Publication Type:Journal Article
- Keywords:
acute myeloid leukemia;
chrysin;
venetoclax;
apoptosis;
Akt/NF-κB signaling pathway
- From:
Chinese Journal of Pathophysiology
2025;41(7):1300-1307
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aims to investigate the synergistic cytotoxic effects of chrysin and venetoclax on acute myeloid leukemia(AML)cells and to elucidate the underlying mechanisms.METHODS:Human AML cell lines MV411 and MOLM13 were cultured in vitro and treated with chrysin in combination with venetoclax.Cell viability was as-sessed using the CCK8 assay,while flow cytometry was employed to measure cell cycle distribution and apoptosis rates.Western blot was used to detect the expression of apoptosis-related proteins and protein kinase B(PKB/Akt)/nuclear factor-κB(NF-κB)signaling pathway-related proteins.RESULTS:The results from the CCK8 assay and flow cytometry demon-strated that treatment with 16 and 32 μmol/L chrysin significantly inhibited the viability of AML cells and increased the proportion of cells in G1 phase,as well as the apoptosis rate.Notably,the cells in combination treatment group exhibited a marked reduction in proliferation and an elevated apoptosis rate compared with either chrysin or venetoclax group alone.Western blot analysis indicated that increasing concentrations of chrysin led to an elevation in cleaved poly(ADP-ribose)polymerase(PARP)level,alongside a down-regulation of proteins associated with the Akt/NF-κB signaling pathway.Fur-thermore,the combination treatment significantly up-regulated cleaved PARP level and down-regulated Akt/NF-κB path-way-related proteins compared with the treatment with chrysin or venetoclax alone.CONCLUSION:Chrysin and veneto-clax synergistically inhibit the proliferation of AML cells and promote apoptosis by modulating the Akt/NF-κB signaling pathway.
