The levels of miR-3195, miR-328-5p, and miR-6867-5p after hyperbaric oxygen therapy in patients with traumatic brain injury and their relationships with prognosis
10.3760/cma.j.cn311847-20210329-00103
- VernacularTitle:创伤性脑损伤患者高压氧治疗后血清miR-3195、miR-328-5p和miR-6867-5p水平及其与预后的关系
- Author:
Jie FENG
1
;
Guilong FENG
;
Ke ZHANG
;
Tong YE
;
Xiaodong DU
;
Jiali ZHANG
Author Information
1. 030001 太原，山西医科大学第一医院急诊医学中心
- Publication Type:Journal Article
- Keywords:
Traumatic brain injury;
Hyperbaric oxygen;
MiR-3195;
MiR-328-5p;
MiR-6867-5p;
Prognosis
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(6):723-727
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the changes in miR-3195, miR-328-5p, and miR-6867-5p expressions levels and their relationships with the prognosis of patients with traumatic brain injury after hyperbaric oxygen (HBO) therapy.Methods:A total of 124 patients with traumatic brain injury admitted to the First Hospital of Shanxi Medical University from May 2018 to August 2020 were selected as research subjects. According to the scores of Glasgow Outcome Scale (GOS) six months after onset, patients with traumatic brain injury were divided into good prognosis group ( n=75) and poor prognosis group ( n=49). The expression levels of MiR-3195, miR-328-5p, and miR-6867-5p before HBO treatment (T1) and after one month of HBO treatment (T2) were detected by real-time fluorescence quantitative PCR. Receiver operating characteristics (ROC) curve and Logistic regression were used to assess the efficiencies of these three indicators in evaluating the prognosis of patients given HBO therapy for traumatic brain injury and their relationships with prognosis. Results:The Glasgow coma scale(GCS) scores of the good prognosis group were higher than those of the poor prognosis group, and the time from onset to treatment was shorter than that of the poor prognosis group, the differences were statistically significant ( P<0.05). The relative expression levels of miR-3195 at T1 and T2, miR-328-5p at T2, and miR-6867-5p at T1 and T2 in the good prognosis group were lower than those in the poor prognosis group ( P<0.05). The relative expression levels of miR-3195, miR-328-5p, and miR-6867-5p at T2 were all lower than those at T1 in both groups ( P<0.05). The relative expression levels of miR-6867-5p in both groups were all reduced, yet the reduction in the good prognosis group was greater than that in the poor prognosis group ( P<0.05). The areas under the curve (AUC) of miR-3195, miR-328-5p, and miR-6867-5p at T2 were bigger than those at T1, and the differences were all statistically significant ( P<0.05). GCS was an independent protective factor for the prognosis of traumatic brain injury ( P<0.05), while the time from onset to treatment and the expression levels of miR-3195 and miR-6867-5p at T2 were independent risk factors for the prognosis of traumatic brain injury ( P<0.05). Conclusion:The expression levels of miR-3195 and miR-6867-5p are highly related to the prognosis of patients with traumatic brain injury after HBO treatment, while the expression level of miR-328-5p is not related to the prognosis.
