Effect of Huangqi-Danggui mixture on neural cell pyroptosis in cerebral ischemia/reperfusion rats
10.3969/j.issn.1000-4718.2025.07.003
- VernacularTitle:黄芪-当归合剂对脑缺血再灌注大鼠神经细胞焦亡的影响
- Author:
Ruikun WANG
1
;
Weijuan GAO
;
Xianming HOU
;
Zhifeng XING
;
Luyao LIU
;
Chengxuan CHAI
;
Yi ZHANG
Author Information
1. 河北中医药大学,河北 石家庄 050091;河北省心脑血管病中医药防治研究重点实验室,河北 石家庄 050091
- Publication Type:Journal Article
- Keywords:
Huangqi-Danggui mixture;
cerebral ischemia-reperfusion injury;
pyroptosis;
neuroprotection
- From:
Chinese Journal of Pathophysiology
2025;41(7):1267-1274
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effects of Huangqi-Danggui mixture(HQDG)on the pyroptosis of brain tis-sues in rats with middle cerebral artery occlusion/reperfusion(MCAO/R),and to explore the mechanism of neuroprotec-tion provided by HQDG.METHODS:Sixty-four SD rats were randomly divided into 4 groups:sham group,model group,HQDG group,and Xuesaitong(XST)group.The infarct volume of brain tissues was observed by 2,3,5-triphenyl-tetrazolium chloride staining,while hematoxylin-eosin staining was used to observe the pathological changes of brain tis-sues.Immunofluorescence was employed to assess the expression of nucleotide-binding oligomerization domain-like recep-tor protein 3(NLRP3),cleaved caspase-1 and gasdermin D(GSDMD)in the ischemic penumbra of brain tissues.The se-rum levels of interleukin-1β(IL-1β)and IL-18 were measured using ELISA.Western blot was used to detect NLRP3,cleaved caspase-1,apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),IL-1β and IL-18 in brain tissues.RESULTS:Compared with sham group,the neurological deficit scores of the rats in model group were significantly increased(P<0.01),while those in HQDG and XST groups were significantly reduced compared with model group(P<0.01).The cerebral infarct volume ratio was significantly reduced in HQDG and XST groups compared with model group(P<0.01).The pathological damage of brain tissue in HQDG and XST groups was significantly reduced compared with model group.The positive rates of NLRP3,cleaved caspase-1 and GSDMD in the ischemic penumbra of brain tissues were significantly decreased in HQDG group compared with model group(P<0.01).The expression of pyrop-tosis-related proteins,NLRP3,cleaved caspase-1 and ASC,in the ischemic penumbra of brain tissues was significantly el-evated in model group compared with sham group(P<0.01),and significantly decreased in HQDG group compared with model group(P<0.01).The serum levels of IL-1β and IL-18 were significantly increased in model group compared with sham group(P<0.01),and significantly reduced in HQDG group compared with model group(P<0.01).CONCLU-SION:The HQDG effectively attenuates brain tissue injury in rats with MCAO/R,and its mechanism may be related to the inhibition of neural cell pyroptosis.
