Regulation of secretion by luteolin via AMPK/GSK3β pathway in rats with polycystic ovary syndrome
10.13699/j.cnki.1001-6821.2024.23.012
- VernacularTitle:木犀草素通过AMPK/GSK3β通路调节多囊卵巢综合征大鼠内分泌的研究
- Author:
Lu LU
1
;
Xiao-ling FENG
;
Kun MA
Author Information
1. 黑龙江中医药大学临床医学院,黑龙江哈尔滨 150000
- Publication Type:Journal Article
- Keywords:
luteolin;
polycystic ovary syndrome;
5'-adenosine monophosphate-activated protein kinase/glycogen synthase kinase 3β pathway;
insulin resistance
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(23):3424-3428
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of luteolin on hormone levels and insulin resistance in rats with polycystic ovary syndrome(PCOS)based on 5'-adenosine monophosphate-activated protein kinase(AMPK)/glycogen synthase kinase 3β(GSK3β)pathway.Methods PCOS model rats were constructed by intragastric administration of letrozole.After successful modeling,rats were randomly divided into model group,control group and experimental-L,experimental-M,experimental-H groups,with 10 rats in each group.Another 10 normal rats were selected as the normal group.experimental-L,experimental-M,experimental-H groups were given luteolin at 20,30 and 40 mg·kg-1,respectively.The control group was given metformin by gavage at 300 mg·kg-1.Both normal group and model group were given 0.9％NaCl by intragastric administration.6 groups of rats were given the drug once a day for 28 days.Fasting blood glucose(FBG)and fasting insulin(FINS)were measured by automatic biochemical analyzer.The expression levels of AMPK and GSK3β in ovarian tissue were measured by Western blot.Results The FBG levels of experimental-M group,experimental-H group,control group,model group and normal group were(6.72±1.50),(5.15±1.04),(5.66±0.68),(13.75±1.09)and(4.22±0.14)mmol·L-1,respectively;the FINS levels were(10.03±2.28),(7.07±0.97),(6.64±0.82),(18.37±2.07)and(5.31±1.05)mU·L-1,respectively;the phospho-AMPK/AMPK ratios were 0.83±0.06,1.15±0.06,1.18±0.04,0.37±0.12 and 1.41±0.12,respectively;the relative expression levels of GSK3β protein were 0.73±0.03,0.39±0.07,0.33±0.12,1.45±0.04 and 0.23±0.03,respectively.Compared with the model group,the above indexes in the experimental-M and experimental-H groups and control group were statistically significant(all P＜0.05).Conclusion Luteolin can significantly improve hormone levels,regulate insulin resistance,and improve ovarian oxidative damage and pathological changes in PCOS rats.The mechanism may be related to the regulation of AMPK/GSK3β pathway.
