Exploring the Protective Effect of Danggui Shaoyao Powder on Ovarian Function in Cyclophosphamide Induced Premature Ovarian Failure Rats Based on AMPK/mTOR Signaling Pathway
10.3870/j.issn.1672-0741.24.11.012
- VernacularTitle:基于AMPK/mTOR信号通路探究当归芍药散对环磷酰胺诱导的卵巢早衰大鼠卵巢功能的保护作用
- Author:
Shangjia WANG
1
;
Dan LI
;
Luyan LU
Author Information
1. 河南中医药大学,郑州 450046
- Publication Type:Journal Article
- Keywords:
AMPK/mTOR signaling pathway;
cyclophosphamide;
Danggui Shaoyao powder;
premature ovarian fail-ure;
ovarian function
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(1):80-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Danggui Shaoyao powder on ovarian function in cyclophospha-mide induced premature ovarian failure rats by regulating the adenosine monophosphate-activated protein kinase(AMPK)/mam-malian target of rapamycin(mTOR)signaling pathway.Methods Seventy-two rats were randomly divided into control group,model group,low-dose Danggui Shaoyao powder(L-Danggui Shaoyao powder,6.11 g/kg)group,medium dose Danggui Shaoyao powder(M-Danggui Shaoyao powder,12.22 g/kg)group,high-dose Danggui Shaoyao powder(H-Danggui Shaoyao powder,24.44 g/kg)group,and H-Danggui Shaoyao San+AMPK agonist AICAR(H-Danggui Shaoyao powder+AICAR,24.44 g/kg Danggui Shaoyao powder+200 mg/kg AICAR)group,with 12 rats in each group.The number of follicles at different stages was observed.ELISA,HE staining,TUNEL kit,and Western blot were used to detect FSH,E2,AMH,LH levels,ovarian tissue pathological changes,cell apoptosis,and expression of AMPK/mTOR pathway related proteins.Results Compared with Con-trol group,the FSH,LH,number of blocked follicles,apoptosis rate,Bax,Caspase-3,and p-AMPK/AMPK protein expression were higher in model group,while E2,AMH,primordial,primary,secondary,and antral follicles,Bcl-2,and p-mTOR/mTOR protein expression were lower(all P＜0.05).Compared with model group,FSH,LH,number of blocked follicles,apoptosis rate,Bax,Caspase-3,and p-AMPK/AMPK protein expression were lower in L,M,and H-Danggui Shaoyao powder groups,while E2,AMH,primordial,primary,secondary,and antral follicles,Bcl-2,and p-mTOR/mTOR protein expression were higher(all P＜0.05).Compared with the H-Danggui Shaoyao powder group,FSH,LH,number of blocked follicles,apoptosis rate,Bax,Caspase-3,and p-AMPK/AMPK protein expression were higher in H-Danggui Shaoyao powder+AICAR group,while E2,AM H,primordial,primary,secondary,and antral follicles,Bcl-2,and p-mTOR/mTOR protein expression were lower(allP＜0.05).Conclusion The improvement of ovarian function in cyclophosphamide induced premature ovarian failure rats by Dang-gui Shaoyao powder may be related to AMPK/mTOR pathway inhibition.
