Expression of NPM3 in Lung Adenocarcinoma and Its Impact on the Biological Function of Lung Adenocarcinoma Cells
10.3870/j.issn.1672-0741.24.07.036
- VernacularTitle:NPM3在肺腺癌中的表达及其对肺腺癌细胞生物学功能的影响
- Author:
Yun WANG
1
;
Yang SHU
;
Xiaobai WU
Author Information
1. 江苏大学附属医院呼吸与危重症医学科,镇江 212001;江苏大学附属医院中心实验室,镇江 212001
- Publication Type:Journal Article
- Keywords:
NPM 3;
lung adenocarcinoma;
epithelial-mesenchymal transition;
cell proliferation;
cell migration
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(1):36-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Nucleophosmin 3(NPM3)in lung adenocarcinoma and its impact on the biological functions of the lung adenocarcinoma cell line H 292,with the goal of providing innovative insights for the diagno-sis and therapeutic strategies of lung adenocarcinoma.Methods The differential expression of the NPM3 gene between lung ad-enocarcinoma and normal controls was analyzed,along with its correlation with the clinicopathological characteristics and prog-nosis of lung adenocarcinoma patients,using the TCGA bioinformatics database.A cell model with reduced NPM3 expression(si-NPM3-H292)was developed by gene interference technology,and the efficiency of this knockdown was assessed by real-time fluorescence quantitative PCR(qRT-PCR)and Western blotting.The impact of NPM3 knockdown on the proliferative capacity of lung adenocarcinoma cells was evaluated by using CCK-8 and plate clone formation assays.The scratch and Transwell assays were employed to assess the effect of NPM 3 knockdown on the migratory potential of H 292 cells.The mRNA and protein ex-pression levels of markers associated with epithelial-mesenchymal transition(EMT)were determined by qRT-PCR and Western blotting.Additionally,the expression levels of proteins involved in the PI3K/Akt signaling pathway were examined by Western blotting.Results Analysis of the TCGA database indicated that NPM 3 is highly expressed in lung adenocarcinoma and is sig-nificantly associated with the clinicopathological classification of lung adenocarcinoma patients.The survival outcomes for pa-tients with high NPM 3 expression were notably worse than those with low expression(P＜0.05).The knockdown cell model was successfully established,and the proliferation and migration capabilities of H292 cells were significantly diminished after NPM3 knockdown(P＜0.05).Compared to the control group,mRNA and protein levels of E-cadherin were increased in si-NPM 3-H292 cells,while the expression level of N-cadherin was reduced at both mRNA and protein levels(P＜0.05).Although no significant change was observed in the protein expressions of Akt and PI3K in si-NPM3-H292 cells compared to the control,there was a decrease in phosphorylation levels,accompanied by an increase in protein expression of PTEN(P＜0.05).Conclusion NPM3 is highly expressed in lung adenocarcinoma,which is associated with poor prognosis of patients.NPM3 may facilitate the proliferation and migration of lung adenocarcinoma cells through regulation of the PI 3K/Akt signaling pathway.
