Experimental study of hyperbaric oxygen therapy on Alzheimer’s disease rat models
10.3760/cma.j.cn311847-20200118-00025
- VernacularTitle:高压氧治疗对阿尔茨海默病模型动物影响的实验研究
- Author:
Bin LUO
1
;
Xiaoyun ZHANG
1
;
Peifu WANG
1
Author Information
1. 100049 北京，航天中心医院神经内科
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Alzheimer’s disease;
Brain-derived neurotrophic factor;
Tropomyosin receptor kinase B;
Nerve growth factor
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(4):450-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of hyperbaric oxygen (HBO) on cognitive function and expression levels of brain-derived neurotrophic factor (BDNF), tropomyosin receptor kinase B (TrkB), nerve growth factor (NGF), and synaptophysin (SYN) in hippocampal tissues of rat models with Alzheimer’s disease (AD).Methods:A total of 80 adult male SD rats were randomly divided into four groups: normal control group, sham operation group, model group, and HBO group, with 20 rats in each group. The normal control group did not receive any treatment; in the sham operation group, the hippocampus on each side of the rat brains was injected with 5 μl of 0.9% sodium chloride solution; in the model group, the hippocampus of rats was injected with 5 μl of β-amyloid 25-35 (Aβ25-35); and in the HBO group, the hippocampus of rats was also injected with 5 μl of Aβ25-35, which was given HBO intervention two weeks after the injection. After the HBO intervention, each group underwent Morris water maze experiment to detect the learning and memory ability of rats and the expressions of BDNF, TrKB, NGF, and SYN in their hippocampi.Results:The escape latency of rats in each group gradually shortened with the extension of training time. There was no significant difference in the escape latency between the normal control group and the sham operation group at each time point (all P>0.05), but on the 5th and 6th day, the escape latency in the HBO group was significantly shorter than that in the model group (all P<0.05), but there was no significant difference when the HBO group was compared with the normal control group or the sham operation group (all P>0.05). There was no significant difference between the normal control group and the sham operation group in the percentage of time spent in the original platform quadrant and the times of rats crossing the original platform (all P>0.05), but the percentage and the times in the two groups were both significantly higher than those in the model group (all P<0.05). The percentage of time spent in the original platform quadrant and the times of rats crossing the original platform in the HBO group were significantly higher than those in the model group (all P<0.05), but there was no significant difference when the HBO group was compared with the normal control group or the sham operation group (all P>0.05). The relative expressions of BDNF, TrkB, NGF protein, and SYN optical density in the normal control group were not significantly different from those in the sham operation group (all P>0.05), but they were significantly different from those in the model group (all P<0.05). The relative expressions of BDNF, TrkB, NGF protein, and SYN optical density in the HBO group were significantly higher than those in the model group (all P<0.05), but there was no significant difference when the HBO group was compared with the normal control group or the sham operation group (all P>0.05). Conclusion:HBO can alleviate the cognitive impairment of AD rat model by upregulating the expressions of BDNF, TrkB, NGF, and SYN, which are closely related to memory.
