Protective effect of exercise preconditioning on kidney injury of rats caused by one-off high intensive and exhaustive military training
10.3760/cma.j.cn311847-20210202-00042
- VernacularTitle:预适应训练对高强度一次性力竭军事训练肾损伤大鼠的防护研究
- Author:
Hong CHEN
1
;
Jing CHEN
;
Tingting HU
;
Lili GAO
;
Xiuzhu LIN
;
Yuqi ZHANG
;
Xiaochen BAO
Author Information
1. 100037 北京，解放军总医院第六医学中心干部诊疗科
- Publication Type:Journal Article
- Keywords:
Exercise preconditioning;
Kidney injury;
Rats;
Military medicine
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(3):360-364,369
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of exercise preconditioning with different intensities and durations on early kidney injury indicators in rats by constructing a rat model of kidney injury induced by one-off high intensive and exhaustive military training, and to provide guidance on implementing scientific training plans.Methods:A total of 70 male Sprague Dawley (SD) rats were divided into seven groups with 10 rats in each group according to the random number table method, namely, model group with kidney injury induced by one-off high-intensive military training (M group), low-intensive and short-term exercise preconditioning group (LS group), low-intensive and long-term exercise preconditioning group (LL group), medium-intensive and short-term exercise preconditioning group (MS group), medium-intensive and long-term exercise preconditioning group (ML group), high-intensive and short-term exercise preconditioning group (HS group), and high-intensive and long-term exercise preconditioning group (HL group). After treadmill exercise preconditioning in different modes, the rats were trained as the kidney injury model group receiving one-off high intensive and exhaustive military training. The behavioral characteristics of rats after each training and when they were trained to exhaustion, the time to exhaustion, the levels of blood urea nitrogen (Bun), serum creatinine (SCr), cystatin C (Cys-C), kidney injury molecule-1(KIM-1), and neutrophil gelatinase-associated lipocalin (NGAL), the activities of Na + K + -ATPase in kidney tissues, the levels of total superoxide dismutase (SOD), and malondialdehyde (MDA), and the semi-quantitative scores of renal tubules were observed. Results:The rats in the HL group had indifferent expressions, slow responses, glossless fur, and weak responses for evading capture, while the rats in other groups had glossy fur and were alert to surrounding stimuli. In terms of the length of the time to exhaustion, the groups were ranked as ML>HS>MS>LL>LS>M>HL. There was no statistically significant difference in the time to exhaustion between the LS group and the M group ( P>0.05); but there were statistically significant differences in the time to exhaustion among other groups, and the time to exhaustion in the HL group was significantly shorter than those in other groups ( P<0.05). In terms of serum Bun, SCr, Cys-C, KIM-1, and NGAL levels, there was no statistically significant difference between the LS group and the M group ( P>0.05), but there were statistically significant differences among other groups ( P<0.05). In terms of the expressions of Na + , K + -ATPase activities and total SOD activity in renal tissues of rats, the groups were ranked as ML>HS>MS>LL>M>LS>HL. In terms of the expressions of Na + , K + -ATPase activities and total SOD activity in renal tissue of rats, there was no significant difference between the M group and the LS group ( P>0.05), but there were statistically significant differences among other groups ( P<0.05). The MDA levels from low to high were ranked as ML0.05), but there were statistically significant differences among other groups ( P<0.05). The renal tubules scores from low to high were ranked as ML
