IL-6 dynamic changes during the sepsis development and main organs damages in the early stage of sepsis in mice
10.3760/cma.j.cn311847-20200717-00290
- VernacularTitle:小鼠脓毒症进程中IL-6的动态变化及其前期主要脏器损伤
- Author:
Wenjie HAN
1
;
Zhaomin TIAN
;
Shihui ZHANG
;
Haitao TIAN
Author Information
1. 100048 北京，解放军总医院第六医学中心
- Publication Type:Journal Article
- Keywords:
Sepsis;
Mouse;
IL-6;
Organ damage
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(2):233-235
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the dynamic changes of IL-6 during the sepsis development and the main organ damages in the early stage of sepsis in mice.Methods:A total of 30 C57 male mice aged 7-8 weeks were randomly divided into control group and sepsis 0, 4, 8, 12, 16 h groups with 5 mice in each group. The mice in each sepsis group were pretreated by lipopolysaccharide (LPS) with 400 μg/kg for 8 hours, and then stimulated by LPS with 10 mg/kg to establish sepsis models. The control group was only given saline with 1 ml/kg by tail vein injection without any other treatment. Enzyme linked immunosorbent assay (ELISA) was used to measure the serum IL-6 content and observe its concentration changes; meanwhile, the liver, kidney, heart, lung, spleen, and other organs of sepsis model mice were taken for pathological tissue sections to explore the pathological changes in the sepsis development process.Results:The levels of serum IL-6 were (239.7±21.5), (1 268.5±48.7), (1 836.8±67.4), (2 393.9±68.3), (2 989.5±84.7) pg/ml in the sepsis 0, 4, 8, 12, 16 h groups, which were significantly higher than those in the control group (56.5±1.8) pg/ml. The results of pathological examination showed that in the early stage of sepsis, the organ damages firstly occurred in heart and liver, while the kidney, lung, and spleen were less affected.Conclusion:As the sepsis in model mice become severe, the serum IL-6 level increases and the heart and liver firstly show organ damages among all the main organs.
