Inhibitory effect of fentanyl combined with hyperbaric oxygen on gastric cancer AGS cells in nuder mice
10.3760/cma.j.cn311847-20210203-00043
- VernacularTitle:芬太尼联合高压氧处理对裸鼠胃癌AGS细胞的抑制作用
- Author:
Guolong JIA
1
;
Yan LI
;
Yanhong HE
;
Tao YANG
;
Guosun DENG
Author Information
1. 730050 兰州，甘肃省中医医院麻醉科
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Gastric cancer;
Apoptosis;
AGS cells
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(3):304-308
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the inhibitory effect of fentanyl combined with hyperbaric oxygen (HBO) on the proliferation of tumor tissues of the gastric cancer tissues of nude mice.Methods:A total of 20 six-week-old male specific pathogen free (SPF) BALB/c nude mice were used to establish a model of transplanted tumors and then were divided into four groups according to the experimental requirements: control group, HBO group, FEN group, and HBO+ FEN group. The control group was raised normally after successful modeling; the HBO group inhaled oxygen for 60 min every day at 0.20 MPa; the FEN group was given 10 mg/kg fentanyl by intragastric administration; and the HBO+ FEN group inhaled oxygen for 60 min at first at 0.20 MPa and then was given 10 mg/kg fentanyl by intragastric administration. All groups were treated every other day for a total of 21 days. After treatment, the nude mice were executed to strip the tumor of which the volume and weight were measured. Terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) assay was applied to detect the apoptosis of tumor cells and Western blotting was adopted to detect the expressions of apoptosis-related proteins and signaling pathway protein.Results:After 21 days of treatment, the tumor volume and weight of the mice in the HBO+ FEN group were significantly lower than those of the other three groups with statistically significant differences ( P<0.05). The number of apoptotic cells of transplanted tumors in the HBO+ FEN group was significantly higher than those of the other three groups with statistically significant differences ( P<0.05). Compared with the control group, the FEN group, and the HBO group, the expression levels of Bax, Caspase-3, and Cytochrome C in transplanted tumor tissues of nude mice in the HBO+ FEN group were significantly higher, while the expression of Bcl-2 protein was significantly lower, with statistically significant differences ( P<0.05). Compared with the control group, the HBO group, and the FEN group, the expression level of p65 protein in transplanted tumor cells in the HBO+ FEN group was significantly lower, while the expression level of p53 protein was significantly higher, with statistically significant differences ( P<0.05). Conclusion:The combination of HBO and fentanyl can promote tumor cell apoptosis by regulating NF-κB and p53 signaling pathways in nude mice with gastric cancer.
