MTERFD1 promotes the proliferation of ovarian cancer cells through regulating IL-6
10.3760/cma.j.cn311847-20210225-00067
- VernacularTitle:MTERFD1通过调节IL-6促进卵巢癌细胞增殖的分子机制研究
- Author:
Ziqi LIU
1
;
Shengyun CAI
1
;
Yu CHEN
1
Author Information
1. 200433 上海，海军军医大学附属长海医院妇产科
- Publication Type:Journal Article
- Keywords:
Ovarian cancer;
MTERFD1;
Proliferation;
IL-6
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(2):197-202,212
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of mitochondrial transcription termination factor domain 1 (MTERFD1) in regulating the malignant biological behavior of ovarian cancer (OC) cells and preliminarily reveal its molecular mechanism.Methods:RT-qPCR was used to detect the expression levels of MTERFD1 mRNA in human OC cell lines SKOV3, HEY, and OVCAR3, human embryonic kidney epithelial cells HEK-293, and normal ovarian tissues. After transfecting these cells with MTERFD1 overexpression plasmid and small interfering RNA, MTT analysis was used to detect the cell proliferation, and Annexin V/PI double staining assay was used to detect apoptosis. ELISA was used to check the effect of MTERFD1 level on the expression of IL-6. MTT analysis was used to evaluate the effect of IL-6 level changing on the proliferation of OC cells with different MTERFD1 expressions.Results:The MTERFD1 mRNA levels in OC cell lines SKOV3, HEY, and OVCAR3 were higher than those in HEK-293 cell line and normal ovarian tissues with statistically significant differences ( P<0.05). In human OC OVCAR3 and HEK-293 cell lines, the cell proliferation in the group of MTERFD1 overexpression plasmid was higher than that in the blank plasmid group; after the MTERFD1 being knocked out, the HEY and SKOV3 cell proliferations obviously decreased and the apoptosis rates were higher than those in the negative control group; all differences were statistically significant ( P<0.05). The IL-6 levels in OC cells were positively correlated with MTERFD1 expressions, and the IL-6 blocking antibody could reverse the promoting effect of MTERFD1 on the proliferation of OC cells. Conclusion:The promoting effect of MTERFD1 on the proliferation of ovarian cancer cells may be achieved through regulating IL-6.
