Effects of hyperbaric oxygen on the hypothalamic-pituitary-adrenal axis in rats with acute carbon monoxide poisoning
10.3760/cma.j.cn311847-20201022-00402
- VernacularTitle:高压氧对急性一氧化碳中毒大鼠下丘脑-垂体-肾上腺轴的作用研究
- Author:
Jingzi ZHANG
1
;
Chao PENG
;
Ya LI
;
Wenlan WANG
Author Information
1. 710032 西安，空军军医大学学员二大队
- Publication Type:Journal Article
- Keywords:
Acute CO poisoning;
Hypothalamic-pituitary-adrenal axis;
Hyperbaric oxygen
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(1):1-4,38
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of hyperbaric oxygen (HBO) on the hypothalamic-pituitary-adrenal (HPA) axis in rats with acute carbon monoxide (CO) poisoning.Methods:Rat models with acute CO poisoning were established and treated with HBO for 7 days. RNA-sequencing (RNA-seq) was used to detect genes in the brain tissue of each group of rats, and real time quantitative PCR (RT-PCR) was adopted to analyze the pro-opiomelanocortin (POMC) of the genes that were differentiated by RNA-seq; the enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of rat serum adrenocorticotropic hormone (ACTH) and cortisol (COR); and immunohistochemistry (IHC) and western blot were applied to detect the expressions of POMC protein and ACTH protein receptor (ACTHR) in rats’ brain.Results:The results of RNA-seq and RT-PCR showed that 7 days after CO poisoning, the POMC gene expression in hypothalamus was significantly increased ( P<0.05), and its expression was downregulated by HBO treatment ( P<0.05). The results of IHC and western blot showed that, compared with the control group, POMC expressions in the cerebral cortex of the CO group were increased ( P<0.05), and POMC expressions in the HBO group were decreased ( P<0.05). ACTHR expressions were increased in the cerebral cortex of the CO group ( P<0.05), but there was no significant difference between the HBO group and the CO group ( P<0.05). The testing results showed that the levels of serum ACTH and COR in the CO group were significantly increased ( P<0.05), and were decreased after HBO treatment ( P<0.05). Conclusion:Acute CO poisoning can activate the HPA axis through the POMC-ACTH-COR pathway, and HBO may exert a therapeutic effect by inhibiting the excessive activation of related genes, proteins, and hormones on the HPA axis.
