Protective effect and mechanism of hyperbaric oxygen therapy on cardiac function in model rats with myocarditis
10.3760/cma.j.cn311847-20201118-00430
- VernacularTitle:高压氧处理对心肌炎模型大鼠心功能的保护作用及其作用机制
- Author:
Yuhui LI
1
;
Haipeng JIANG
;
Xiaoning SUN
;
Qiang LI
;
Mei LYU
;
Jinhe CHEN
;
Qing SUN
;
Shuai LI
;
Hongtao NIU
;
Song ZHANG
Author Information
1. 264000 山东省烟台，烟台山医院
- Publication Type:Journal Article
- Keywords:
Myocarditis;
Rats;
Hyperbaric oxygen;
Cardiac function
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2021;28(1):69-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect and mechanism of hyperbaric oxygen (HBO) on cardiac function in model rats with myocarditis.Methods:A total of 30 specific pathogen free (SPF) male SD rats aged 4~5 weeks were randomly divided into three groups with 10 rats in each group, which were the control group, the model group, and the HBO group. The control group was only given 1 ml/kg normal saline by tail vein injection without any other treatment; the rat models of myocarditis in the model group and the HBO group were established by injecting lipopolysaccharide (LPS) via tail vein; the HBO group was additionally treated with HBO. After 6 days of treatment, echocardiography was obtained by ultrasound imaging system for small animals; and the data of left ventricular ejection fraction (EF), fraction shortening (FS), and maximum ascending and descending rate (+ dP/dt max) were collected. The levels of serum interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), brain natriuretic peptide (BNP), and catalase (CAT) were detected by enzyme-linked immunosorbent assay (ELISA) kit. After euthanasia, the myocardial tissue was taken. Some of them was stained by Hematoxylin and Eosin (HE) for morphological observation; the rest was ground for detecting the expression levels of oxidative stress related factors, including superoxide dismutase (SOD) and malondialdehyde (MDA), by ELISA. Results:The results of echocardiography showed that EF, FS, + dP/dt max, and - dP/dt max in the model group were significantly lower than those in the control group ( P<0.05); after HBO treatment, EF, FS, + dP/dt max, and -dP/dt max in the HBO group were significantly higher than those in the model group ( P<0.05). The results of ELISA showed that IL-6, IL-1 β, TNF-α, and BNP in the model group were significantly higher than those in the control group ( P<0.05); after HBO treatment, IL-6, IL-1β, TNF-α, and BNP in the HBO group were significantly lower than those in the model group ( P<0.05). Under light microscope, there was no focal infiltration of inflammatory cells in the myocardial tissue of the control group. The cardiomyocytes of the model group were arranged disorderly with more inflammatory cell infiltration. And some pink protein mucus could be seen in some blood vessels. In the HBO group, inflammatory cell infiltration was milder than that in the model group. And there were some edematous cardiomyocytes. Compared with the control group, the expression levels of SOD in myocardial tissue and CAT protein in the serum of rats with myocarditis in the model group were significantly decreased, while the expression level of MDA in myocardial tissue was significantly increased ( P<0.05). After HBO treatment, compared with the model group, the expression levels of SOD in myocardial tissue and CAT protein in the serum of rats with myocarditis in the HBO group were significantly increased, while the expression of MDA in myocardial tissue was significantly decreased ( P<0.05). Conclusion:HBO treatment can improve the cardiac function in model rats with myocarditis by inhibiting the inflammatory response and alleviating oxidative stress damage.
