Effect of Xianglian Pills on Panapoptotic Pathway in Ulcerative Colitis Mice
10.3870/j.issn.1672-0741.24.03.030
- VernacularTitle:香连丸对溃疡性结肠炎小鼠泛凋亡途径的影响
- Author:
Hongxia HE
1
;
Heng FAN
;
Jia YANG
Author Information
1. 华中科技大学同济医学院附属协和医院中医科,武汉 430022;武汉市中西医结合医院消化内科,武汉 430014
- Publication Type:Journal Article
- Keywords:
Xianglian pill;
PANoptosis;
ulcerative colitis
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2024;53(6):717-722
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of PANoptosis in the pathogenesis of ulcerative colitis(UC),and to study the regulatory mechanism of Xianglian pills on panapoptotic pathway related proteins in UC mice.Methods Forty-eight C57BL/6 mice were randomly divided into 6 groups:normal group,model group,mesalazine group,low,medium and high dose of Xiangli-an pills group.Except the normal group,the other 5 groups were given 3％dextran sulfate(DSS)for 1 week,respectively,to pre-pare experimental UC mice model.At the same time,starting from the first day of modeling,the corresponding groups were giv-en mesalazine or Xianglian pills by intragastric administration,respectively,for continuous treatment for 1 week.On the 8th day,colon tissues of mice in each group were taken and partially fixed for HE staining and pathological observation.TUNEL method was used to detect the apoptosis of colon tissue cells,and the contents of IFN-γ,TNF-α,IL-1β and IL-6 in colon were detected by enzyme-linked immunosorbent assay(ELISA).Western blot was used to detect the expression of Bax,Bcl-2,Caspase-8,p-MLKL,p-RIPK3,Caspase-1,GSDMD-N and IL-1β in colon tissues.Results Compared with model group,disease activity index(DAI)and degree of colon tissue damage decreased in high-dose Xianglian pills group(both P＜0.01),and the levels of IFN-γ,TNF-α,IL-1β and IL-6 in UC mice decreased(all P＜0.01),and colon lengths and body weights increase(both P＜0.01).In ad-dition,apoptosis of UC mice was inhibited in the high-dose group by downregulating Bax/Bcl-2 ratio(P＜0.01),and necroptosis was inhibited by increasing Caspase-8 protein level and decreasing p-MLKL and p-RIPK3 protein levels(all P＜0.01).Pyropto-sis was inhibited by decreasing the expression levels of Caspase-1,GSDMD-N and IL-1β(all P＜0.01).Conclusion Xianglian pills can relieve DSS induced ulcerative colitis in mice by regulating apoptosis,necroptosis and pyroptosis.
