Adenovirus mediated Profurin expression improved the plaque stability of ApoE-/-mice
10.20039/j.cnki.1007-3949.2025.04.003
- VernacularTitle:腺病毒介导Profurin表达改善ApoE-/-小鼠斑块稳定性
- Author:
Xinyi ZHANG
1
;
Chen WANG
;
Wanyue YANG
;
Ze YANG
;
Guodong REN
;
Jiguo ZHANG
;
Yang YU
;
Ke ZHANG
Author Information
1. 山东第一医科大学(山东省医学科学院)附属省立医院急诊科,山东省济南市 250021;山东第一医科大学(山东省医学科学院)实验动物学院(山东省实验动物中心),山东省济南市 250117;山东第一医科大学(山东省医学科学院)临床与基础医学院,山东省济南市 250117
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
plaque stability;
lipoprotein;
phospholipid transfer protein;
Profurin
- From:
Chinese Journal of Arteriosclerosis
2025;33(4):297-302
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of adenovirus(ADV)-mediated Profurin(PF)expression on the plaque stability of ApoE-/-mice.Methods ApoE-/-mice were fed with high-fat diet for 8 weeks,and then treated with ADV-mediated PF intervention,followed by high-fat diet for 4 weeks.Aortic roots were isolated for atherosclerotic plaque area analysis and immunohistochemical analysis.Plasma phospholipid transfer protein(PLTP)activity was detec-ted by fluorescence donor essay,plasma total cholesterol(TC)and triglyceride(TG)were measured by enzyme assay kits,and fast protein liquid chromatography was used for lipoprotein profile analysis.Results Compared with the con-trol group,the plasma TC and TG levels,PLTP activity and circulating tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels in ADV-PF group were significantly decreased(P＜0.01).In the ADV-PF group,there was no significant change in atherosclerotic lesions on the inner surface of the full-length aorta,but the plaque area and lipid area in the aortic root were reduced(P＜0.01),the content of macrophages was significantly decreased(P＜0.01),and the smooth muscle cells and collagen area were not significantly different.The content of matrix metalloproteinase-9 in plaque was signifi-cantly decreased(P＜0.05).Conclusion Overexpression of PF can alleviate atherosclerosis and reduce the levels of circulating inflammatory factors to a certain extent,and effectively improve the plaque stability of ApoE-/-mice.
