Changes of p-ERK expression in rat lungs with decompression sickness caused by fast buoyancy ascent escape
10.3760/cma.j.cn311847-20200414-00147
- VernacularTitle:快速上浮脱险致减压病早期大鼠肺组织细胞外调节蛋白激酶活性变化研究
- Author:
Jun MA
1
;
Yiqun FANG
;
Xiaochen BAO
;
Fangfang WANG
;
Na LI
;
Pu YOU
;
Shi ZHANG
;
Haiting CHEN
Author Information
1. 200433 上海，海军军医大学海军特色医学中心
- Publication Type:Journal Article
- Keywords:
Fast buoyancy ascent escape;
Lung injury;
Decompression sickness;
p-ERK
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(3):283-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of inflammatory response in lung tissues of rats with decompression sickness (DCS) caused by fast buoyancy ascent escape, and to observe the changes in phosphorylation of extracellular signal-related kinase (p-ERK) expression in rats with early onset of DCS during a simulated fast buoyancy ascent escape.Methods:A total of 18 rats were divided into three groups randomly, i. e. control group (C group), escape group in which all six rats were dead (ED group), and escape group in which all six rats survived (ES group). The rats in the ES group were anesthetized and sacrificed 8 minutes after they managed the simulated insecure seawater fast buoyancy ascent escape in a hyperbaric chamber. The p-ERK protein was extracted from rat lungs and then the protein expression was analyzed by Western blotting.Results:The p-ERK expression level in the ED group was elevated significantly than that in the C group; while the p-ERK expression level in ES group was not significantly increased than that in the C group.Conclusion:The development of inflammation-like reaction in lung tissues of rats with DCS caused by fast buoyancy ascent escape may relate with the activation of p-ERK.
