Effects of oleanolic acid on diabetic nephropathy and NF-κB/caspase-9 signaling pathway in rats
10.13431/j.cnki.immunol.j.20250011
- VernacularTitle:齐墩果酸对糖尿病肾病大鼠及NF-κB/caspase-9信号通路的影响
- Author:
Qianqian ZHAI
1
;
Chengliang SHEN
;
Yan LI
;
Meng CAO
;
Tao WANG
;
Yan QIN
;
Yunfeng ZHU
Author Information
1. 453100 卫辉,新乡医学院第一附属医院内分泌科
- Publication Type:Journal Article
- Keywords:
Oleanolic acid;
Diabetic nephropathy;
NF-κB/caspase-9 signaling pathway;
Renal tissue
- From:
Immunological Journal
2025;41(2):80-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of oleanolic acid on NF-κB/caspase-9 signaling pathway in kidneys of rat with diabetic nephropathy.Methods The diabetic nephropathy model rats were established,and the model rats were randomly divided intooleanolic acid low,medium and high dose groups,metformin group,and model group,with another 12 healthy SD rats as control group.The levels of blood glucose were measured at weeks 1,2 and 3 post-drug administration,and blood lipid and 24h urine urinary microalbumin(UMA)were measured after entire drug administration.Furthermore,we also detected the renal histopathology of rats,apoptosis of renal tubular,glomerular cells,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and protein expression of NF-κB/caspase-9 signaling pathway.Results Compared with the control group,the model group demonstrated higher levels of blood glucose at weeks 1,2 and 3,blood lipid,UMA,TNF-α and IL-6,higher proportion of apoptotic renal tubular and glomerular,and higher expressions of caspase-9 protein and p-NF-κB p65/NF-κB p65(P＜0.05).Compared with the model group,the pathological damage of renal tissue in the metformin group and oleanolic acid low,middle and high dose groups were alleviated,the levels of blood glucose at weeks 1,2 and 3,blood lipid,UMA,TNF-α and IL-6,the proportion of apoptotic renal tubular and glomerular,and the expressions of caspase-9 proteins and p-NF-κB p65/NF-κB p65 were decreased,and these indexes in oleanolic acid groups showed a dose-dependent manner(P＜0.05).Conclusion Oleanolic acid can improve the metabolism of glucose and lipid,reduce the pathological damage of renal tissue,inhibit the apoptosis of renal tubular and glomerular,and inhibit the NF-κB/caspase-9 pathway in diabetic nephropathy rats.
