Effect of hyperbaric oxygen on proliferation of endogenous neural stem cells in rats with focal cerebral ischemia
10.3760/cma.j.cn311847-20190918-00229
- VernacularTitle:高压氧对局灶性脑缺血大鼠内源性神经干细胞增殖的影响
- Author:
Yongjun MAI
1
;
Xiang LI
;
Liangzuo ZHENG
;
Hong YANG
;
Xiaomou WEI
;
Lianbi XUE
Author Information
1. 545005 广西壮族自治区柳州，广西医科大学第四附属医院
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Focal cerebral ischemia;
Neural stem cells
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(3):286-290
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of hyperbaric oxygen (HBO) at different courses of treatment on the proliferation of endogenous neural stem cells(NSCs) in cerebral ventricular subventricular zone (SVZ) and hippocampal dentate gyrus (DG) in rats with focal cerebral ischemia at different periods.Methods:The model of focal cerebral ischemia-reperfusion was performed by middle cerebral artery occlusion (MCAO). A total of 105 rats were divided by lottery into pseudo-surgery group(SS), ischemia-reperfusion group (IR group), HBO at 1 st day after ischemia-reperfusion group (IR1d+ HBO group), HBO at 7 th day after ischemia-reperfusion group (IR7d+ HBO group), HBO at 14 th day after ischemia-reperfusion group (IR14d+ HBO group), and HBO at 28 th day after ischemia-reperfusion group (IR28d+ HBO). The SVZ and DG-derived NSCs were detected by immunofluorescence BrdU/Nestin double labeling at the 2 nd, 4 th, 6 th, and 8 th week after modeling. Results:At the 2 nd week after modeling, the number of DG BrdU/Nestin positive cells was 21.20±2.58 in the SS group, 56.40±4.51 in the IR group, 82.80±7.19 in the IR1d+ HBO group, and 70.00±5.09 in the IR7d+ HBO group. At the 4 th week, it was 21.00±2.92 in the SS group, 37.20±3.27 in the IR group, 70.60±5.12 in the IR1d+ HBO group, 57.20±3.56 in the IR7d+ HBO group, 45.80±4.32 in the IR14d+ HBO group. At the 6 th week, it was 20.20±1.92 in the SS group, 26.40±2.74 in the IR group, 48.00±3.16 in the IR1d+ HBO group, 40.60±3.36 in the IR7d+ HBO group, 31.60±2.41 in the IR14d+ HBO group, 26.60±2.30 in the IR28d+ HBO group. The number of DG BrdU/Nestin positive cells was statistically significant among all subgroups during the same period ( P<0.01) except for that at the 8 th week. The pairwise comparison showed the numbers of DG BrdU/Nestin positive cells in the IR group at all time points were all higher than those in the SS group at the same time point ( P<0.05). Except for that at the 8 th week, all the numbers of DG BrdU/Nestin positive cells in the IR1d+ HBO group, the IR7d+ HBO group, and the IR14d+ HBO group at each time point were higher than those in the IR group ( P<0.05). In all the subgroups, the number of DG BrdU/Nestin positive cells reached the highest point at the 2 nd week/the initial measurement point ( P<0.05). At the 2 nd week after modeling, the number of SVZ BrdU/Nestin positive cells was 25.20±2.86 in the SS group, 66.40±2.96 in the IR group, 90.40±6.50 in the IR1d+ HBO group, 75.00±4.58 in the IR7d+ HBO group. At the 4 th week, it was 24.20±1.48 in the SS group, 49.80±4.32 in the IR group, 72.40±4.92 in the IR1d+ HBO group, 57.80±6.46 in the IR7d+ HBO group, 43.80±3.56 in the IR14d+ HBO group. At the 6 th week, it was 24.40±2.41 in the SS group, 28.80±2.77 in the IR group, 51.00±4.30 in the IR1d+ HBO group, 42.00±3.39 in the IR7d+ HBO group, 34.80±2.58 in the IR14d+ HBO group, 31.30±3.41 in the IR28d+ HBO group. The number of SVZ BrdU/Nestin positive cells was statistically significant among all subgroups during the same period ( P<0.05). The pairwise comparison showed the numbers of SVZ BrdU/Nestin positive cells in the IR group at all time points were all higher than those in the SS group at the same time point ( P<0.05) except for the 8 th week. The numbers of SVZ BrdU/Nestin positive cells in the IR1d+ HBO group at each time point were higher than that in the IR group( P<0.05). The numbers of SVZ BrdU/Nestin positive cells in the IR7d+ HBO group, the IR14d+ HBO group, and the IR28d+ HBO group at the 2 nd, 4 th, and 6 th week were higher than those in the IR group at the same time point ( P<0.05). In all the subgroups, the number of SVZ BrdU/Nestin positive cells reached the highest point at the 2 nd week/the initial measurement point ( P<0.05). Conclusion:The earlier HBO treatment on cerebral infarction starts, the better promotion of the proliferation of SVZ and DG-derived NSCs achieves. HBO treatment has almost no effect on the proliferation of endogenous NSCs at late cerebral infarction.
