Study on the Mechanism of the Intervention of Yiqi Jianpi Jiedu Compound on HBx-Mediated Liver Cancer Stem Cells from the Perspective of PI3K/AKT Pathway
10.11842/wst.20240910007
- VernacularTitle:从PI3K/AKT途径探讨益气健脾解毒复方对HBx介导肝癌干细胞干预作用的机制研究
- Author:
Zhulin WU
1
;
Sen LIN
;
Weijun LUO
;
Siyi LI
;
Weiqing ZHANG
;
Lanyue MA
;
Chunshan WEI
;
Lisheng PENG
Author Information
1. 深圳市龙华区人民医院 深圳 518000
- Publication Type:Journal Article
- Keywords:
Yiqi Jianpi Jiedu;
Qizhu Xiaozheng Fang;
Phyllanthus urinaria L.;
Liver cancer stem cells;
PI3K/Akt
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(4):939-949
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of the intervention effect of Qizhu Xiaozheng Fang(QZXZF),a representative prescription of Yiqi Jianpi Jiedu(replenishing qi,strengthening spleen,and removing toxicity),in treating HBx-mediated liver cancer stem cells(LCSCs).Methods The Kaplan-Meier survival analysis was used to investigate the effects of Yiqi Jianpi Jiedu on the prognosis of patients with HBV-HCC.The network pharmacology method was utilized to predict the targets and pathways of QZXZF in treating HBx-related LCSCs(HBx-LCSCs).HBx-LCSCs cells were screened by stably transfecting HBx and serum-free culture.The therapeutic effect of QZXZF on HBx-LCSCs was tested in vitro,and its effect on stemness markers and PI3K/Akt pathway was verified by qRT-PCR and Western blot.Results Yiqi Jianpi Jiedu could improve the overall survival time of HBV-HCC patients.Combined with the results of network pharmacology,the mechanism of action of QZXZF against HBx-LCSCs was explored from the PI3K/Akt pathway.Compared with the blank vector group,HBx can promote the expression of stemness markers in HBx-LCSCs cells;compared with the HBx-LCSCs control group,QZXZF could significantly inhibit the proliferation and colony formation of HBx-LCSCs cells(in a concentration-dependent manner),and could reduce the expression of stemness markers(EpCAM,NANOG,SOX2,and OCT4),phosphorylated PI3K(p-PI3K)and phosphorylated AKT(p-AKT)proteins.Conclusion QZXZF may regulate HBx-mediated LCSCs through PI3K/AKT pathways,providing a reference for the mechanism of TCM intervention in LCSCs.
