Clinical application value of fluorescence in situ hybridization in detecting circulating tumor cells in patients with breast cancer
10.13602/j.cnki.jcls.2025.05.03
- VernacularTitle:荧光原位杂交技术检测乳腺癌患者循环肿瘤细胞的临床应用价值
- Author:
Yanyu CHEN
1
;
Xiang CHEN
1
Author Information
1. 江苏大学附属宜兴医院甲状腺乳腺外科,江苏宜兴 214200
- Publication Type:Journal Article
- Keywords:
breast cancer;
circulating tumor cell;
TNM staging;
molecular typing
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(5):332-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical significance and application value of circulating tumor cells(CTCs)detected by fluo-rescence in situ hybridization(FISH)in the diagnosis of breast cancer.Methods A total of 114 breast cancer patients and 30 patients with benign breast lesions admitted to the Department of Thyroid and Breast Surgery,Yixing Hospital Affiliated to Jiangsu University from January 2014 to June 2022 were enrolled.Their peripheral blood cells were enriched by the immunomagnetic bead negative enrich-ment method,and the levels of CTCs were detected by FISH technology.The diagnostic efficacy of CTCs in breast cancer was evaluated by the receiver operating characteristics(ROC)curve,and the correlations of the levels of CTCs with clinicopathological parameters of breast cancer patients were analyzed by the chi-square analysis.Results The detection results of FISH showed that 75 of 114 breast cancer patients were positive for CTCs and 39 were negative.The evaluation results of the ROC curve for the diagnostic efficacy of CTCs in breast cancer showed that when the cut-off value was 2.5 CTCs/mL,its sensitivity and specificity were 52.0%and 89.7%,respec-tively,and the area under the ROC curve(AUCROC)was the best(0.68).There were significant differences in the positive rates of CTCs among different TMN stages and molecular types of breast cancer patients(P＜0.05),but not among different vascular invasion types and immunohistochemical indicators of breast cancer patients(P＞0.05).Conclusion The positive of CTCs detected by FISH may indicate different TNM staging and poor molecular typing.The CTCs is expected to become an important biomarker affecting the clinical decision-making and prognosis evaluation in the treatment of early breast cancer.
