Expression levels and clinical significance of serum exosomal LncRNA GAS5 in gastric cancer patients
10.13602/j.cnki.jcls.2025.05.04
- VernacularTitle:血清外泌体LncRNA GAS5在胃癌患者中的表达水平及其临床意义
- Author:
Xiangqian ZHANG
1
;
Xiao HAN
;
Kun ZHANG
Author Information
1. 河南电力医院检验科,郑州 450052
- Publication Type:Journal Article
- Keywords:
gastric cancer;
exosome;
serum;
LncRNA GAS5;
biomarker;
diagnosis
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(5):336-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression levels of serum exosomal LncRNA GAS5 in gastric cancer(GC)patients and eval-uate its clinical significance.Methods Seventy GC patients admitted to Henan Electric Power Hospital from January 2019 to Decem-ber 2020 and 70 age-and sex-matched healthy controls undergoing physical examination during the same period were enrolled.Their se-rum samples and clinicopathological data were collected.Additionally,cancer tissue and adjacent normal tissue specimens were ob-tained from 20 GC patients who underwent surgical resection.The expression levels of LncRNA GAS5 in tissues and serum exosomes were detected by real-time fluorescence quantitative PCR(qRT-PCR).The chemiluminescence method was used to detect the levels of serum carcinoembryonic antigen(CEA)and carbohydrate antigen 19-9(CA19-9).The receiver operating characteristic(ROC)curve was used to assess the diagnostic efficacy of different detection indicators for GC.The correlations of serum exosomal LncRNA GAS5 levels with clinicopathological parameters and prognosis of GC patients were also analyzed.Results The expression levels of serum ex-osomal LncRNA GAS5 in GC patients were significantly lower than that in healthy controls(t=10.84,P＜0.001).The expression levels of LncRNA GAS5 in GC tissues were also significantly lower than that in adjacent tissues(t=14.94,P＜0.001).The analysis results of the ROC curve showed that the area under the ROC curve(AUCROC)of serum exosomal LncRNA GAS5 for the screening of GC was 0.807.When the cut-off value was 1.96,its sensitivity was 81.43%and specificity was 67.14%.The diagnostic efficacy of serum exoso-mal LncRNA GAS5 for GC was significantly better than traditional tumor markers CEA and CA19-9,and their AUCROC were 0.664 and 0.713,respectively.The analysis results of clinicopathological parameters of GC patients showed that low serum exosomal LncRNA GAS5 levels were significantly correlated with large tumor size,advanced TNM stage,and distant metastasis(P＜0.05).The overall survival time of patients with low expression of serum exosomal LncRNA GAS5 was significantly lower than that of patients with high ex-pression(median overall survival time:23 vs 35 months,P=0.03).Conclusion Serum exosomal LncRNA GAS5 in GC patients is down-regulated,which has good diagnostic efficacy for GC and is expected to become a potential biological marker for the diagnosis and prognosis evaluation of GC patients.
