Functional Analysis of the Promoter of the deSUMOylation Enzyme senp8 and Its Relationship with Lipid Metabolism in Yellow Catfish(Pelteobagrus fulvidraco)
10.13865/j.cnki.cjbmb.2025.02.1231
- VernacularTitle:黄颡鱼去SUMO化修饰关键基因senp8启动子的功能分析及其与脂质代谢的关系
- Author:
Fu-Xuan DUAN
1
;
Zhi-Wei HAO
1
;
Tao LIU
1
;
Chang-Chun SONG
1
;
Tian-Hua ZHANG
1
;
Chong-Chao ZHONG
1
;
Xiao-Ying TAN
1
Author Information
1. 华中农业大学水产学院营养系,水产动物分子营养实验室,武汉 430070
- Publication Type:Journal Article
- Keywords:
Pelteobagrus fulvidraco;
DeSUMOylation;
promoter;
lipid metabolism;
transcriptional regu-lation
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(4):607-616
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the transcriptionally regulatory mechanism of the senp8 promoter in yellow cat-fish(Pelteobagrus fulvidraco);this study used P.fulvidraco as the research subject.Dual-luciferase re-porter assay and electrophoretic mobility shift assay were employed to analyze the functional activity of the promoter;coupled with in vivo experiments.The results indicated that the 2 045 bp senp8 promoter se-quence contained key transcription factor binding sites such as SP1;TATA-Box;CCAAT-Box;SREBP1;PPARα;and PPARγ.The binding sites of SREBP1(-901/-910 bp);PPARα(-1 291/-1 308 bp);and PPARγ(-1 292/-1 306 bp)in the senp8 promoter positively regulate its activity;and oleic acid or palmitic acid promote this binding.Furthermore;high-fat feeding promoted the expression of the senp8 gene and its protein in the liver of P.fulvidraco;oleic acid or palmitic acid treatment significantly en-hanced the activity of the senp8 promoter;and this enhancement could be achieved through the regulatory effects of SREBP1;PPARα;and PPARγ response elements.Additionally;high-fat feeding influenced the mRNA and protein expression levels of genes related to deSUMOylation modification in the liver of P.fulvidraco.This study provides new insights into the relationship between deSUMOylation modification and the regulation of lipid metabolism in the vertebrates.
