Different Effects of Acupuncture at Different Time on the Expression of TRPV1 and SP in Neuropathic Pain Mice
- VernacularTitle:择时针刺对神经病理性疼痛小鼠TRPV1和SP表达的不同调整作用
- Author:
Linghuo ZENG
1
;
Han JIANG
1
;
Dingjun CAI
1
Author Information
- Publication Type:Journal Article
- Keywords: Acupuncture at different time; Neuropathic pain; TRPV1; Substance P
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(2):592-600
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the effect of acupuncture at different time on dorsal root ganglion(DRG)in neuropathic pain(NP)mice.Different regulatory effects of transient receptor potential vanilloid 1(TRPV1)and substance P(SP)in DRG.Methods A total of64 C57BL/6J mice were randomly divided into two intervention groups:ZT8 group and ZT16 group,among which two intervention groups were randomly divided into control group(8 mice),sham-operation group(8 mice),model group(8 mice)and acupuncture group(8 mice).The chronic constriction injury(CCI)model was prepared by sciatic nerve ligation in model group and acupuncture group,and only the sutured wound after sciatic nerve was exposed in sham-operation group.On the 8th day after operation,acupuncture treatment was performed on mice in the acupuncture group.The intervention methods were the same in the two groups,only the acupuncture time was different.The"Yanglingquan"on the surgical side was taken at the acupoint,and manual acupuncture and needle were performed once a day for 15 minutes each time.The mechanical pain and thermal pain thresholds of each group of mice were measured at 3,7,10 and 14 days after modeling to evaluate their pain.The expression of SP and TRPV1 in the L4-6 DRG on the surgical side of mice was observed by immunofluorescence double labeling method,and the SP content in the tissue around the sciatic nerve on the affected side and the skin on the dorsal fibra of the foot was determined by enzymed-linked immunosorbent assay(ELISA).Results ① Acupuncture intervention could improve mechanical and thermal hyperalgesia in CCI mice.And ZT8 time point acupuncture effect is better than ZT16 time point.②Acupuncture can significantly reduce the expression of TRPV1 and SP in DRG of CCI mice,and the effect of acupuncture at ZT8 time point is better than that at ZT16 time point.③Acupuncture can significantly reduce the SP concentration in the peripheral tissue of CCI mice,and the effect of acupuncture at ZT8 time point is better than that at ZT16 time point.④Acupuncture could significantly reduce the SP concentration in the distal skin of the injured nerve in CCI mice,and there was no significant difference between ZT8 and ZT16.Conclusion Acupuncture at different time has different regulation effects on hyperalgesia in CCI mice,and the regulation effect of ZT8 is better than that of ZT16.This may be related to the different regulatory effects of acupuncture at different time on the expression of TRPV1 and SP in DRG of CCI mice and the local SP concentration of injured nerves.
