Berberine Activates SGK1 to Protect MyocardialIinjury Induced by Sunitinib
- VernacularTitle:小檗碱激活SGK1保护舒尼替尼导致心肌损伤
- Author:
Congxin LI
1
;
Xuejing LI
1
;
Suhui QIE
1
;
Wei YAN
1
;
Yuan GAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Sunitinib; Myocardial injury; Human induced pluripotent stem cell-derived cardiomyocytes; Neonatal rat cardiomyocytes; Berberine; Serum glucocorticoid regulated kinase 1
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(2):384-390
- CountryChina
- Language:Chinese
- Abstract: Objective At the level of human induced pluripotent stem cell-derived cardiomyocytes(hiPSC-CMs)and neonatal rat cardiomyocytes(NRVMs),investigate the role of serum glucocorticoid regulated kinase 1(SGK1)in the myocardial injury caused by sunitinib and the potential protective effect of berberine.Methods Enzyme-linked immunosorbent assay was used to detect the effect of sunitinib and incubation with berberine on the level of NT-proBNP and cTn-I in hiPSC-CMs.The effect of sunitinib and incubation with berberine on the activity of SGK1 in hiPSC-CMs was quantitatively tested using kinase activity photometry.Western blot was used to detect the expression of SGK1 in NRVMs.And the above indicators were detected again after SGK1 blockade using SGK1 blocker.Results Sunitinib and SGK1 inhibitor significantly increased the NT-proBNP and cTn-I level of hiPSC-CMs(P<0.01),and incubation with berberine activated SGK1 could significantly protect hiPSC-CMs from the increase in NT-proBNP level caused by sunitinib(P<0.01),while the SGK1 inhibitor resisted the protective effect of berberine(P<0.01).Sunitinib and SGK1 inhibitor significantly decreased the activity of SGK1 in hiPSC-CMs(P<0.01),and incubation with berberine activated SGK1,which significantly protected hiPSC-CMs from the inhibition of SGK1 activity caused by sunitinib(P<0.01).Sunitinib and SGK1 inhibitors significantly reduced SGK1 protein expression in NRVMs(P<0.01),and incubating berberine to activate SGK1 significantly protected SGK1 protein expression decrease induced by sunitinib(P<0.01).Conclusions Sunitinib inhibits the activity of SGK1,leading to myocardial injury,while berberine activates SGK1 and has protective effects.
