Screening of IgG N-glycosylation markers associated with ankylosing spondylitis
10.3760/cma.j.cn141217-20230913-00080
- VernacularTitle:强直性脊柱炎相关免疫球蛋白G N-糖基化标志物的筛选
- Author:
Xin WEN
1
;
Jia YIN
;
Aihong ZHOU
;
Lei TAO
;
Zhangshen RAN
;
Wenyan LUO
;
Shuqi LIU
;
Guoyong DING
;
Daiyu SONG
Author Information
1. 山东第一医科大学公共卫生与健康管理学院流行病学研究室，济南 270117
- Publication Type:Journal Article
- Keywords:
Spondylitis, ankylosing;
Immunoglobulin G;
Glycosylation;
Biomarkers
- From:
Chinese Journal of Rheumatology
2025;29(1):25-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the potential of IgG N-glycans as diagnostic biomarker for ankylosing spondylitis (AS) by comparing and analyzing the IgG N-glycan profiles with AS and healthy controls.Methods:A 1∶1 matched case-control study design was adopted, 81 AS patients who visited the Department of Rheumatology and Immunology at Taian City Central Hospital and the Second Affiliated Hospital of Shandong First Medical University between July 2020 and June 2021 were recruited. These patients were matched with 81 healthy individuals undergoing routine physical checkup. The levels of IgG N-glycosylation in human plasma were quantitatively measured using ultrahigh-performance liquid chromatography. Binomial logistic regression analysis was performed to identify IgG N-glycan biomarkers associated with AS.Results:A total of 14 primary glycans and 13 derived traits showed statistically significant differences between the AS case group and the control group. Binomial logistic regression analysis showed that glycan peak 4, agalactosylated glycans, fucosylated glycans, and fucosylated agalactosylated glycans were positively associated with AS[ OR(95% CI)=1.12(1.01, 1.42), 1.21(1.03, 1.43), 1.48(1.08, 2.03), and 1.27(1.04, 1.55); P=0.036, 0.022, 0.039, 0.020, respectively]. In terms of diagnostic performance, the single glycan GP4 exhibited the largest area under the ROC curve, with an AUC (95% CI) 0.751 (0.677, 0.826), while the combined glycan indicators (GP4+G0+F+FG0) achieved an AUC (95% CI) 0.768(0.697, 0.840). Conclusion:IgG N-glycans have the potentials to serve as candidate biomarkers for AS, and warrants further investigation.
