Study on Peripheral Blood CD4 and CD8 T-lymphocyte ATP Release Assays for the Assessment of Immune Function in Patients with Gastrointestinal Tumors
10.3969/j.issn.1671-7414.2025.03.027
- VernacularTitle:外周血CD4和CD8 T淋巴细胞ATP释放试验用于消化道肿瘤患者免疫功能评估研究
- Author:
Xihan WANG
1
;
Yao QIN
;
Qinyi ZHANG
;
Hongbin ZHANG
Author Information
1. 南方医科大学第一临床医学院,广州 510515;中国人民解放军南部战区总医院基础医学实验室,广州 510010
- Publication Type:Journal Article
- Keywords:
immune function;
gastrointestinal tumors;
CD4 T lymphocyte;
CD8 T lymphocyte;
ATP release assay
- From:
Journal of Modern Laboratory Medicine
2025;40(3):145-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of CD4 and CD8 T lymphocyte ATP release assays for the assessment of cellular immune function in patients with digestive tract tumors.Methods 51 digestive tract tumor patients from the Department of Oncology and Department of Radiotherapy of the General Hospital of Southern Theater Command of PLA during 2023～2024 as a study group,and 51 healthy medical checkups were collected from the Physical Examination Center of that Hospital during 2018～2020 as a control group,and the counts of leukocytes,lymphocytes,CD4 T lymphocyte,and CD8 T lymphocyte were conducted to detect the release of ATP from CD4,CD8 T lymphocyte before and after the stimulation of PHA Levels of ATP release from CD4 and CD8 T lymphocytes before and after PHA stimulation.Results Lymphocyte counts[1.45(1.12,1.75)×109/L]and CD8 T lymphocyte counts[357.00(232.13,451.35)个/μl]in the research group was lower than in the control group[2.04(1.24,2.27)×109/L,454.36(368.00,566.10)个/μl],and the differences were statistically significant(Z=-4.719,-3.209,all P<0.05).After PHA stimulation,the ATP release level of CD4 T lymphocytes in the research group was lower than that in the control group(Z=-2.181,P＜0.05),and the level of ATP release level of CD8 T lymphocytes in the research group before and after PHA stimulation were lower than those in the control group(Z=-3.314,-3.462,all P<0.05).There was no correlation between CD4 T lymphocyte ATP release values and CD4 T lymphocyte counts in the research group(r=0.617,P=0.093),and there was a positive correlation between CD4 T lymphocyte ATP release values and CD4 T lymphocyte counts in the control group(r=0.276,P=0.049).There was no significant correlation between the ATP release values of CD8 T lymphocytes and lymphatic counts in the research group(r=0.033,P=0.816),and a positive correlation between the ATP release values of CD8 T lymphocytes and lymphatic counts in the control group(r=0.278,P=0.048).Conclusion The CD4 and CD8 T lymphocyte ATP release assay can be used as a method to evaluate the dynamic function of cellular immunity,and in combination with static cell counting can provide a more comprehensive assessment of cellular immunity in patients with gastrointestinal tumors.
