Qualitative Analysis of Chemical Components in TangNiaoLing Tablets by UHPLC-Q-Exactive-Orbitrap-MS/MS
- VernacularTitle:基于UHPLC-Q-Exactive-Orbitrap-MS/MS的糖尿灵片成分鉴定
- Author:
Yanzhao ZHANG
1
;
Ying LI
;
Kangya GUO
;
Lei ZHANG
;
Yan LEI
;
Shidan ZANG
;
Qian WANG
;
Hongwei JIANG
Author Information
- Publication Type:Journal Article
- Keywords: TangNiaoLing tablets; UHPLC-Q-Exactive-Orbitrap-MS/MS; Composition characterization; Mass spec-trometry; Diabetes
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(2):391-403
- CountryChina
- Language:Chinese
- Abstract: Objective To determine the chemical composition of TangNiaoLing Tablets by UHPLC-Q-Exactive-Orbitrap-MS/MS.Methods A Waters ACQUITY HSS T3 column(100 mm×2.1 mm,1.8 μm)was used for separation at a total flow rate of 0.2 mL/min.The mobile phase included an aqueous solution of 0.1%formic acid and acetonitrile mixed with 0.1%formic acid was supplied.The injection volume was set at 2 μL and the column oven temperature was 40℃.High-resolution mass spectrometric data were obtained by concurrently scanning the positive and negative ion modes.The identification was accomplished by inferring the empirical fragmentation patterns and comparing it with databases and references.Results 100 different chemical elements,including triterpenes,flavonoids,phenylpropanoids,phenylethanoid glycosides,iridoid glycosides,and phenols,among others were identified from the 50%methanol extract of TangNiaoLing pills.Conclusion The chemical contents of TangNiaoLing tablets were identified and analyzed using the UHPLC-Q-Exactive-Orbitrap-MS/MS method for the first time.This served as a foundation for future research into the tablets' effective components and quality control.
