Comparative study of high-risk human papillomavirus E6/E7 mRNA detection and pathological examination of cervical lesions
10.3760/cma.j.cn115455-20240102-00001
- VernacularTitle:高危型人乳头瘤病毒E6/E7 mRNA检测与宫颈病变病理检查结果对照研究
- Author:
Zehua ZHAO
1
;
Hua WANG
1
;
Mei MA
1
;
Jinping NIU
1
;
Zhaomin JIANG
1
Author Information
1. 定州市人民医院妇一科，定州 073000
- Publication Type:Journal Article
- Keywords:
Cervicalintraepithelial neoplasia;
High-risk human papillomavirus;
Pathological conditions, signs and symptoms;
Diagnosis
- From:
Chinese Journal of Postgraduates of Medicine
2025;48(6):506-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the clinical significance of high risk human papillomavirus (HR-HPV) positive female cervical lesion screening HR-HPVE6/E7 mRNA detection.Methods:A total of 80 HR-HPV-positive female patients with cervical lesions were retrospectively selected in Dingzhou People′s Hospital from August 2021 to August 2023. According to the pathological examination results, the patients were divided into inflammatory response group (16 cases), cervical intraepithelial neoplasia (CIN) Ⅰ group (15 cases), CINⅡ group (22 cases), CINⅢ group (17 cases) and cervical cancer group (10 cases). The viral load and positive rate of HR-HPVE6/E7 mRNA in the five groups were compared. Receiver operating characteristic (ROC) curve was drawn to analyze the diagnostic efficacy of HR-HPVE6/E7 mRNA in cervical lesions.Results:The viral load of HR-HPVE6/E7 mRNA in the cervical cancer group, CINⅢ group, CINⅡ group, CINⅠ group and inflammatory response group was gradually decreased: 3 152.62(894.26, 21 986.24), 812.62(465.05, 3 945.06), 481.64(215.62, 2 451.49), 38.62(0, 612.47), 0(0, 316.28) × 10 3 copy/L, there was statistical difference among the five groups ( P<0.05). The positive rate of HR-HPVE6/E7 mRNA in cervical cancer group (10/10) was higher than that in CINⅢ group (10/17), CINⅡ group [27.27%(6/22)], CINⅠ group (2/15) and inflammatory response group (0), and there were statistical differences ( P<0.05). ROC curve analysis results showed that the area under the curve of HR-HPVE6/E7 mRNA for diagnosis of cervical lesions was 0.901(95% CI 0.837 - 0.942), sensitivity was 92.53%, specificity was 89.12%. Conclusions:Quantitative detection of HR-HPVE6/E7 mRNA viral load has high sensitivity and specificity in the diagnosis of cervical lesions.
