Effects of remazolam toluene sulfonate on cognitive dysfunction after partial hepatectomy in elderly rats by regulating the CX3CL1-CX3CR1 signaling pathway
10.13431/j.cnki.immunol.j.20240123
- VernacularTitle:甲苯磺酸瑞马唑仑通过调节CX3CL1-CX3CR1信号通路对老年大鼠肝部分切除术后认知功能障碍的影响
- Author:
Longping CHEN
1
;
Jun LI
1
Author Information
1. 621000,绵阳市中心医院麻醉科
- Publication Type:Journal Article
- Keywords:
Remazolam toluene sulfonate;
Chemokine C-X3-C motif ligand 1/chemokine C-X3-C motif receptor 1 signaling pathway;
Partial hepatectomy;
Postoperative cognitive dysfunction
- From:
Immunological Journal
2024;40(12):883-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of remazolam toluene sulfonate(RT)on cognitive dysfunction and chemokine C-X3-C motif ligand 1/chemokine C-X3-C motif receptor 1(CX3CL1-CX3CR1)signaling pathway in elderly rats after partial hepatectomy.Methods All experimental rats were randomly separated into a Control group,a model group(POCD group),a low-dose and high-dose remazolam toluene sulfonate groups(RT-L,RT-H groups),and a high-dose remazolam toluene sulfonate+CX3CL1 antibody group(RT-H+CX3CL1 antibody group).A partial hepatectomy elderly rat model was established and corresponding drug treatments were given according to the groups.Morris water maze was applied to detect cognitive function in rats.ELISA was applied to detect the levels of inflammatory factors TNF-α and IL-6 in rat serum.Nissl staining was applied to observe the pathological changes of hippocampal neurons in rats.TUNEL staining was applied to detect neuronal cell apoptosis in rats.Western blot was applied to detect the expression of apoptosis related proteins Bax,Caspase-3,C-caspase-3,and CX3CL1-CX3CR1 signaling pathway related proteins.Results RT reduced the escape latency of POCD in aged rats,increased the number of rats crossing the platform,alleviated neuronal damage,basically arranged cells,increased cell number,alleviated nuclear pyknosis,relatively clear nucleolus,decreased TNF-α,IL-6 levels,neuronal apoptosis index and Bax,Caspase-3,C-caspase-3 expression.The expression of CX3CL1 and CX3CR1 was increased,and the effect was more significant at high dose.CX3CL1 antibody can partially reverse the improvement of RT on POCD.Conclusion Remazolam toluene sulfonate can improve cognitive function after partial hepatectomy in elderly rats,and its mechanism of action is related to the activation of the CX3CL1-CX3CR1 signaling pathway.
