Prognostic Value of Cerebrospinal Fluid Sequencing Reads in Varicella-Zoster Virus Encephalitis/Meningitis:An Analysis Based on met-agenomic Next Generation Sequencing
10.11969/j.issn.1673-548X.2025.04.025
- VernacularTitle:基于mNGS的带状疱疹病毒脑炎/脑膜炎预后分析
- Author:
Jingzhe HAN
1
;
Ye JI
1
;
Cuizhi HAN
1
Author Information
1. 053000 河北省哈励逊国际和平医院(衡水市人民医院)神经内科
- Publication Type:Journal Article
- Keywords:
Varicella zoster virus;
Meningitis;
Encephalitis;
Cerebrospinal fluid;
Metagenomic next generation sequencing
- From:
Journal of Medical Research
2025;54(4):141-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective Varicella zoster virus(VZV)is a prevalent etiological agent of viral encephalitis/meningitis,and met-agenomic next generation sequencing(mNGS)has demonstrated its capability to rapidly and accurately diagnose VZV encephalitis/menin-gitis.However,there are limited studies investigating the prognostic value of mNGS sequence reads in cerebrospinal fluid(CSF)for VZV encephalitis/meningitis.In this study,we aimed to identify risk factors associated with poor prognosis in VZV encephalitis/meningitis pa-tients based on mNGS sequence reads,aiming to enhance clinical prediction.Methods A total of 54 patients diagnosed with VZV en-cephalitis,confirmed by cerebrospinal fluid mNGS,were selected from the Department of Neurology at Hengshui People's Hospital be-tween January 2016 and October 2022.Based on the Glasgow outcome scale(GOS)three months post-discharge,patients were catego-rized into a good prognosis group(26 cases)and a poor prognosis group(28 cases).The clinical features,auxiliary examinations,and prognostic outcomes of both groups were analyzed and summarized.Results Statistically significant differences were observed between the two groups in terms of diabetes prevalence,incidence of high fever,time interval between onset of neurological symptoms and initiation of antiviral treatment,leukocyte count in CSF,as well as the mNGS sequence reads(P＜0.05).Multivariate Logistic regression analysis re-vealed that a history of diabetes mellitus,elevated leukocyte levels in CSF,and higher number of VZV sequence reads detected by mNGS in CSF independently contributed to poor prognosis among patients with VZV encephalitis/meningitis(P＜0.05).Conclusion Patients with VZV encephalitis/meningitis who have comorbid diabetes mellitus along with higher leukocyte levels in CSF and increased mNGS se-quence reads exhibited worse prognoses three months after discharge.
