Analysis of the Expression of TCOF1 in Hepatocellular Carcinoma and its Clinical Significance Based on Bioinformatics
10.11969/j.issn.1673-548X.2025.04.019
- VernacularTitle:基于生物信息学分析细胞质核糖体生物发生因子1在肝癌中的表达及意义
- Author:
Xiaocai MEI
1
;
Jian ZHANG
;
Lingling CAO
Author Information
1. 550001 贵阳,贵州中医药大学第二附属医院肝胆胰脾外科
- Publication Type:Journal Article
- Keywords:
TCOF1;
Hepatocellular carcinoma;
Prognosis;
Bioinformatics;
Level of expression;
Clinical significance
- From:
Journal of Medical Research
2025;54(4):102-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of cytoplasmic ribosome biogenesis factor 1(TCOF1)in hepatocellular carcino-ma and its clinical significance by using bioinformatics methods.Methods To analyze the differences in TCOF1 expression in hepatocel-lular carcinoma tissues and normal liver tissues using TCGA is database and to analyze the relationship between TCOF1 expression and clinicopathological parameters and survival prognosis of hepatocellular carcinoma patients.The LinkedOmics online tool was used to ana-lyze the genes associated with TCOF1 co-expression;gene enrichment analysis was used to explore the possible mechanism of the role of TCOF1 in hepatocellular carcinoma.After transfection of hepatocellular carcinoma cells with si-RNA knocking down TCOF1,the effect of TCOF1 inhibition on the proliferation of hepatocellular carcinoma cells was analysed by plate cloning assay,EDU assay.Results TCOF1 expression was significantly higher in hepatocellular carcinoma tissues than in paraneoplastic tissues(P＜0.001).The expression of TCOF1 correlated with gender,age,tumor grade,metastasis,histological type and mutation of TP53 in hepatocellular carcinoma pa-tients(P＜0.05).Overall survival(P＜0.001)and disease-free survival(P＜0.001)were worse in patients with hepatocellular car-cinoma with high expression of TCOF1.The proliferative ability of hepatocellular carcinoma cells was significantly down-regulated after inhibiting the expression of TCOF1 compared with the NC group.TCOF1 was closely associated with multiple genes,and further enrich-ment analysis demonstrated that these genes play important roles in a variety of biological processes.Conclusion TCOF1 expression is in-creased in hepatocellular carcinoma tissues and leads to poor prognosis in hepatocellular carcinoma patients,and it is expected to be a po-tential prognostic marker for hepatocellular carcinoma and a new target for tumor therapy.
