Effect of miR-378-5p on angiogenesis of glioma
10.3969/j.issn.1006-5725.2025.02.005
- VernacularTitle:微小RNA-378-5p对胶质瘤血管生成的影响
- Author:
Jiayi CAI
1
;
Siyu CHEN
;
Nyulue CAI
;
Wende LI
Author Information
1. 广东省实验动物监测所动物部,广东广州 510663;浙江中医药大学第一附属医院药学部,浙江杭州310006;浙江中医药大学临床药学与药事管理学教研室,浙江杭州 310006
- Publication Type:Journal Article
- Keywords:
microRNA-378-5p;
glioma;
angiogenesis;
VEGF-A
- From:
The Journal of Practical Medicine
2025;41(2):186-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-378-5p on glioma angiogenesis and its molecular mechanism. Methods The expression of miR-378-5p were detected by qRT-PCR in four glioma cells. The levels of VEGF were analyzed by ELISA. HUVECs were treated conditioned medium which collected by glioma cells supernatant. The effects of miR-378-5p on the proliferation,migration,invasion,and angiogenesis of HUVECs were detected by CCK-8,Transwell assay,and tubular formation assay. Results U251 was the highest expression of miR-378-5p,while U87 MG was the lowest expression. Glioma-conditioned media increased miR-378-5p levels significantly promoted the proliferation,migration,invasion,and angiogenesis of HUVECs. The results were con-trary with glioma-conditioned media decreased miR-378-5p levels. miR-378-5p promoted the release of VEGF-A from glioma cells. The proliferation,migration,invasion,and angiogenesis of HUVECs treated-VEGF neutralizing antibody were significantly downregulated. Conclusion miR-378-5p promoted HUVECs proliferation,migration,invasion,and angiogenesis by increasing the release of VEGF from glioma cells.
