Effects of long non-coding RNA LSINCT5 regulating miRNA-451 on cell proliferation and migration ability of cervical squamous cell carcinoma
10.3760/cma.j.cn115355-20250110-00026
- VernacularTitle:长链非编码RNA LSINCT5调控miRNA-451对子宫颈鳞状细胞癌细胞增殖、迁移能力的影响
- Author:
Hua BAI
1
;
Na LU
;
Suhui WU
Author Information
1. 山西白求恩医院（山西医学科学院） 山西医科大学第三医院 同济山西医院妇产科，太原 030032
- Publication Type:Journal Article
- Keywords:
Cervical neoplasms;
Long non-coding RNA;
Cell proliferation;
Cell invasion
- From:
Cancer Research and Clinic
2025;37(5):351-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression level of long non-coding RNA long stress-induced non-coding transcript 5 (LSINCT5) in cervical squamous cell carcinoma tissues and its effect of regulating miRNA-451(miR-451) on cell proliferation and migration ability of cervical squamous cell carcinoma and the possible mechanism.Methods:Cancer tissues and its adjacent normal tissues (2 cm away from the tumor edge) of 40 patients with cervical squamous cell carcinoma in Shanxi Province Cancer Hospital and Shanxi Bethunn Hospital from January 2023 to June 2023 were collected. Human normal cervical epithelial cell line H8 and cervical squamous cell carcinoma cell lines SiHa and CaSki were selected. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the relative expression levels of LSINCT5 and miR-451 in cervical cancer tissues, its adjacent tissues and all cell lines. The SiHa cell line with the highest relative expression level of LSINCT5 was selected and transfected with the small interfering RNA targeting LSINCT5 (si-LSINCT5 group) sequence and its negative control sequence (si-Con group), respectively. CCK-8 method was also used to detect the proliferation ability of SiHa cell of both groups. The migration ability of the 2 groups of SiHa cells was detected by using scratch healing assay. Dual luciferase reporter gene assay was used to verify the targeting relationship between LSINCT5 and miR-451. Western blot assay was used to detect the expressions of related proteins in PI3K-AKT-mTOR signaling pathway in the 2 groups.Results:The relative expression level of LSINCT5 in cancer tissues and adjacent tissues of 40 patients with cervical squamous cell carcinoma was 3.25±0.44 and 1.02±0.18, respectively, and the relative expression level in cancer tissues was higher than that in adjacent tissues ( t = 25.69, P < 0.01). The relative expression level of LSINCT5 in normal cervical epithelial cells H8, cervical squamous cell carcinoma cells CaSki and SiHa was 1.00±0.06, 2.41±0.08 and 4.42±0.09, respectively, and the difference was statistically significant ( F = 5.48, P < 0.001). CCK-8 method showed that the proliferation ability of SiHa cells in the si-LSINCT5 group was lower than that in the si-Con group from 24 h after transfection, and the differences were statistically significant (all P < 0.05). The cell scratch healing experiment showed that the cell scratch inhibition rates of the si-LSINCT5 group and the si-Con group were (70±6)% and (34±9)%, respectively, and the differences were statistically significant ( t = 5.76, P < 0.05). Dual luciferase reporter gene assay confirmed that LSINCT5 had a targeting relationship with miR-451. The relative expression levels of p-PI3K, p-Akt, and p-mTOR in si-LSINCT5 group were lower than those in the si-Con group (all P < 0.05). Conclusions:The relative expression level of lncRNA LSINCT5 is high in cervical squamous cell carcinoma tissues. Knockdown of LSINCT5 may inhibit the proliferation and migration ability of cervical squamous cell carcinoma by sponging miR-451 and reducing the expression of related proteins in PI3K-AKT-mTOR signaling pathway.
