MiR-1 Inhibits Proliferation,Migration and Invasion of Esophageal Squamous Cell Carcinoma
10.11969/j.issn.1673-548X.2025.04.016
- VernacularTitle:miR-1抑制食管鳞状细胞癌细胞增殖、迁移及侵袭
- Author:
Li YAO
1
;
Weichen ZUO
1
;
Siqi GOU
1
Author Information
1. 710018 西北大学附属医院·西安市第三医院病理科
- Publication Type:Journal Article
- Keywords:
miR-1;
Esophageal squamous cell carcinoma;
Proliferation;
Migration;
Invasion
- From:
Journal of Medical Research
2025;54(4):84-88,95
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of miR-1 on the proliferation,migration and invasion of esophageal squamous cell carcinoma.Methods miR-1 mimics,inhibitors and their corresponding controls were transfected into ESCC cells ECa109 and KYSE410.The efficiency of cell transfection was validated by SYBR-green quantitative PCR.The proliferation,migration and invasion ability of ESCC cells were assessed by MTT,foci formation,wound healing and Matrigel invasion assays,respectively.Statistical analysis was carried out using SPSS 22.0software for Windows.Results Overexpression of miR-1 markedly inhibited the cell growth rate and the colony-forming ability of the ESCC cells.While,downregulation of miR-1 promoted the cell growth rate and foci formation(P＜0.05).And upregulation or downregulation of miR-1 significantly suppressed or promoted tumor cell invasion and migration(P＜0.05).Conclu-sion miR-1 inhibited ESCC cell proliferation,invasion and migration,suggesting that miR-1 functions as a tumor suppressor in the tumorigenesis and progression of ESCC and may be a potential therapeutic target.
