Effect of Resveratrol on the Function of Synovial Fibroblasts in Osteoarthritis by Regulating NF-κB Signaling Pathway
10.11969/j.issn.1673-548X.2025.04.027
- VernacularTitle:白藜芦醇调控NF-κB信号通路改善骨关节炎滑膜成纤维细胞功能的实验研究
- Author:
Wen SUN
1
;
Xueyan LI
;
Liyuan ZHANG
Author Information
1. 215000 南京医科大学附属苏州医院麻醉科
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Osteoarthritis;
Synovial fibroblast;
NF-κB signaling pathway
- From:
Journal of Medical Research
2025;54(4):151-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of resveratrol(RES)on the function of osteoarthritis synovioblast(OA-FLS)and its molecular mechanism.Methods The purchased primary synovial cells were cultured to the third generation,and OA-FLS cell model was induced by IL-1β.The activity of FLS cells was observed by CCK-8 assay,and the appropriate RES intervention concentration was selected.The cells were divided into Control group,OA-FLS group,Low-RES group(1μmol/L)and High-RES group(5μmol/L).The effects of RES on the migration and invasion of OA-FLS were detected by cell scratch assay and Transwell assay.The effect of RES on OA-FLS apoptosis was detected by flow cytometry.The effects of RES on the expression of inflammatory factors in OA-FLS and re-lated proteins in NF-κB signaling pathway were detected by Western blot.Results The results of CCK-8 experiment showed that the concentration of RES above 10μmol/L significantly inhibited the viability of FLS cells,and the concentration of RES above 1 μmol/L and 5μmol/L had no significant effect on the viability of FLS cells.Compared with the Control group,the migration and invasion ability of OA-FLS group was enhanced,while the apoptosis ability was weakened,and the migration,invasion and apoptosis ability of OA-FLS group was significantly reversed after RES intervention.Western blot results showed that,compared with the Control group,the levels of inflammatory cytokines in OA-FLS group were increased,and the NF-κB signaling pathway was activated,while the treatment of OA-FLS with different concentrations of RES could reduce the expression of inflammatory cytokines and inhibit the transcription of nuclear fac-tors.Conclusion RES inhibited migration and invasion of OA-FLS,promoted apoptosis,and decreased the expression of inflammatory factors of OA-FLS,which may be related to the inhibition of NF-κB signaling pathway by RES.
