Targeting Ganglionic Macrophages to Improve Post-infarction Arrhythmias
10.11969/j.issn.1673-548X.2025.04.007
- VernacularTitle:靶向神经节巨噬细胞改善心肌梗死后心律失常
- Author:
Hengyang LIU
1
;
Haosong XI
1
;
Hong JIANG
1
Author Information
1. 430061 武汉大学人民医院心内科
- Publication Type:Journal Article
- Keywords:
Macrophage;
Left stellate ganglion;
Clodronate-liposomes;
Arrhythmias
- From:
Journal of Medical Research
2025;54(4):28-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective Exploring the effect of clodronate liposome depletion of left stellate ganglion macrophages on ventricular ar-rhythmias after acute infarction in Beagle dogs.Methods Twelve adult male Beagles were divided into clod and control groups,with the Control group receiving microinjections of phosphate-buffered saline in the left stellate ganglion(LSG)and the Clod group receiving clo-dronate liposome.After modelling of acute cardiac infarction,electrocardiograms were recorded,neural function and activity were meas-ured,heart rate variability scores were analysed,hematoxylin-eosin staining was used to observe pathological changes in cardiomyocyte tissues.Immunofluorescence staining was used to verify the clearance of macrophages by disodium clodronate liposomes and fluorescence quantitative polymerase chain reaction was used to detect the level of expression of nuclear factor-kappa B(NF-κB)-related genes in LSG.Results Ventricular arrhythmias were significantly reduced in the clod group,with statistically significant differences(P＜0.05),significant inhibition of post-infarction LSG overactivation,improvement of heart rate variability,no significant impairment of hematoxylin-eosin staining,significant reduction in macrophages,and decreased expression of genes related to the NF-κB pathway(P＜0.05).Conclusion Clodronate-liposomes improve post-infarction arrhythmias by depletion of left stellate ganglion macrophages.The thera-peutic effect may be through an inhibitory effect on the NF-κB signalling pathway.
