Research Advances in Pathogen Nucleic Acid Point-of-Care Testing Techniques Based on CRISPR-Cas12a System
10.3969/j.issn.1671-7414.2025.01.041
- VernacularTitle:基于CRISPR-Cas12a系统的病原体核酸床旁检测技术方法研究的最新进展
- Author:
Siqi WANG
1
;
Ze WU
1
;
Nan YU
1
Author Information
1. 南方医科大学检验与生物技术学院,广州 510515
- Publication Type:Journal Article
- Keywords:
CRISPR-Cas;
pathogen nucleic acid detection;
POCT
- From:
Journal of Modern Laboratory Medicine
2025;40(1):213-220
- CountryChina
- Language:Chinese
-
Abstract:
Pathogen nucleic acid detection is critical to diagnosing,treating and preventing infectious diseases. To efficiently conduct pathogen nucleic acid detection,the development of point-of-care testing(POCT) with immediacy and simplicity is of great significance. A highly effective and practical gene editing tool discovered for nucleic acid detection is the clustered regularly interspaced short palindromic repeats-CRISPR associated proteins (CRISPR-Cas) systems. Among them,CRISPR-Cas12a is widely used due to its simple structure and powerful functions of target binding and collateral cleavage of gene fragments. Based on the CRISPR-Cas12a system,the pathogen nucleic acid detection method utilizes isothermal and temperature-variable nucleic acid amplification techniques and optical signals as the detection medium for POCT. It features high sensitivity,specificity and simplified detection procedures. This article mainly introduces the latest research progress of pathogen nucleic acid detection POCT techniques based on the CRISPR-Cas12a system,providing new ideas for developing rapid and accurate pathogen nucleic acid detection methods.
