Effect of TUG1 on the Biological Behavior of Human Umbilical Vein Endothelial Cells after Oxygen Glucose Deprivation/Reoxygenation Inju-ry
10.11969/j.issn.1673-548X.2025.01.007
- VernacularTitle:TUG1对氧糖剥夺/复氧损伤后人脐静脉内皮细胞生物学行为的影响
- Author:
Fei LI
1
;
Xinyuan ZHANG
1
;
Hongxiang JIANG
1
Author Information
1. 430060 武汉大学人民医院神经外科
- Publication Type:Journal Article
- Keywords:
Taurine-upregulates gene 1;
Human umbilical vein endothelial cells;
Apoptosis;
Proliferation;
Transfer
- From:
Journal of Medical Research
2025;54(1):31-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of taurine-upregulated gene 1(TUG1)on the biological functions of human um-bilical vein endothelial cells(HUVEC)such as apoptosis,proliferation,and migration after oxygen-glucose deprivation/reoxygenation(OGD/R)injury.Methods HUVEC were treated with OGD/R to establish a cell model injured by OGD/R.Small interfering RNA si-lencing TUG1(si-TUG1)and its negative control(si-NC)were transfected,and the cells were divided into the control group,OGD/R group,OGD/R+si-NC group,and OGD/R+si-TUG1 group.PCR was used to detect the expression level of TUG1 in HUVEC;flow cytometry was used to detect cell apoptosis;CCK-8 assay was used to detect cell proliferation;wound-healing assay was used to detect cell migration;immunofluorescence was used to detect the expression of CD31 in HUVEC;and Western blot was used to detect the expres-sion of nuclear proliferation antigen(PCNA)protein.Results The PCR results showed that the expression level of TUG1 in OGD/R group cells was significantly higher than that in the control group(P＜0.05);si-TUG1 transfection can significantly reduce the expres-sion level of TUG1 in HUVEC(P＜0.05);the apoptosis rate of HUVEC in the si-TUG1 group was significantly lower than that in the si-NC group(P＜0.05);The cell proliferation,cell migration,immunofluorescence intensity of CD31,and the expression levels of PC-NA protein in the si-TUG1 group were higher than those in the si-NC group(P＜0.05).Conclusion Inhibiting TUG1 can significant-ly reduce the apoptosis of HUVEC under OGD/R conditions,and increase the biological functions of HUVEC,such as proliferation,mi-gration,and tubular ability.
