Correlation between Serum LncRNA RMST,miR-582-5p Expression Levels and Early Neurological Deterioration and Prognosis in Patients with Acute Ischemic Stroke
10.3969/j.issn.1671-7414.2025.01.012
- VernacularTitle:急性缺血性脑卒中患者血清LncRNA RMST,miR-582-5p表达水平与早期神经功能恶化及预后的相关性研究
- Author:
Jianhua REN
1
;
Ran ZHANG
;
Xiuying CUI
;
Xin ZHAO
Author Information
1. 承德市中心医院神经诊断科,河北承德 067000
- Publication Type:Journal Article
- Keywords:
long non-coding RNA rhabdomyosarcoma associated transcript-2;
micro RNA-582-5p;
acute ischemic stroke;
neurological deterioration
- From:
Journal of Modern Laboratory Medicine
2025;40(1):64-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of serum long non-coding RNA rhabdomyosarcoma associated transcript-2 (LncRNA RMST) and miR-582-5p in patients with acute ischemic stroke (AIS),and their correlation with early neurological deterioration (END) and prognosis. Methods A total of 129 patients with ischemic stroke admitted to Chengde Central Hospital from January 2021 to December 2022 were gathered,according to diagnostic criteria and patients were divided into END group (n=42) and non END group (n=87) besed on whether END occurred within one week. In addition,59 healthy individuals who underwent physical examination in the hospital were regarded as the health group. The real-time fluorescence quantitative polymerase chain reaction method was applied to detect serum LncRNA RMST and miR-582-5p levels in each group. The Pearson method was used to analyze the correlation between serum LncRNA RMST and miR-582-5p levels in END patients. ROC curve was applied to evaluate further the levels of blood LncRNA RMST and miR-582-5p,and the efficacy analysis of their combination in predicting the prognosis of AIS patients. Results Serum LncRNA RMST(1.01±0.28,2.10±0.41,3.99±0.52) levels gradually increased in the healthy,non-END and END groups,and miR-582-5p(1.02±0.23,0.86±0.16,0.73±0.15) levels gradually decreased,and the differences were statistically significant (F=672.974,31.907,all P<0.05). There was a significant negative correlation between serum LncRNA RMST levels were significantly negatively correlated with miR-582-5p levels (r=-0.451,P<0.001). The AUC(95％CI) of the combined diagnosis of serum LncRNA RMST and miR-582-5p levels in AIS patients was 0.961(0.912～0.987),which was better than the independent prediction (Z=2.280,4.515,all P<0.05). Conclusion The levels of serum LncRNA RMST and miR-582-5p were closely related to END,and they are expected to become predictive factors for the prognosis of AIS patients.
