Study on the Correlation between Serum miR-424,miR-363-3p Levels and Disease Progression and Prognosis in Patients with Multiple Myeloma
10.3969/j.issn.1671-7414.2025.01.008
- VernacularTitle:多发性骨髓瘤患者血清miR-424,miR-363-3p水平表达与病情进展及预后相关性研究
- Author:
Xuan ZHANG
1
;
Jing YANG
1
Author Information
1. 西安市人民医院/西安市第四医院,西安710000
- Publication Type:Journal Article
- Keywords:
multiple myeloma;
miR-424;
miR-363-3p
- From:
Journal of Modern Laboratory Medicine
2025;40(1):43-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between serum levels of miR-424 and miR-363-3p and disease progression in patients with multiple myeloma (MM),and the value of combined prediction of prognosis. Methods A total of 76 patients with MM admitted to Xi'an People's Hospital from January 2022 to September 2023 were included in the study group. There were 19 patients in the International Staging System Phase Ⅰ,27 patients in Phase Ⅰ,and 30 patients in Phase Ⅲ. Immunological classification confirmed 16 cases of IgA type,43 cases of IgG type,11 cases of light chain type,and 6 instances of nonsecretory type. 75 healthy volunteers who underwent physical examinations during the same period were included as the healthy control group. Detected the serum levels of miR-424 and miR-363-3p between groups,perform Logistic factor analysis,and draw receiver operating characteristic (ROC) curves to explore the relationship between miR-424 and miR-363-3p in the progression and prognosis of MM. Results The miR-424 (2.77±0.61) level in the study group was higher than that in the healthy control group (0.83±0.26),and the miR-363-3p level (0.49±0.09) was lower than that in the healthy control group (1.20±0.21),with statistically significant differences (t=25.362,27.064,all P<0.05).The miR-424 level in stage Ⅲ MM patients was significantly higher than that in stage Ⅰ,stage Ⅱ patients (t=11.988,10.366),and miR-363-3p was lower than that in stage Ⅰ,stage Ⅱ MM patients (t=8.077,5.543),with statistically significant differences (all P<0.05).The miR-424 levels in IgG type MM patients were significantly higher than those in IgA type,light chain type,and non secretory type (t=5.967,5.358,3.960),and the miR-363-3p levels were significantly lower than those in IgA type,light chain type,and non secretory type (t=11.594,8.013,6.994),with statistically significant differences (all P<0.05).The levels of Cys-C and miR-424 in MM patients with poor prognosis were significantly higher than those in the good prognosis group,while the levels of miR-363-3p were lower than those in the good prognosis group,with statistically significant differences (t=3.067,6.137,4.011,all P<0.05) .The optimal cut-off values for miR-424 and miR-363-3p in diagnosing MM were 1.73 and 0.40,respectively. The AUC(95％CI) of the combined diagnosis ROC curve[0.911(0.801～0.973)]was higher than that of miR-424[0.792(0.685～0.822)]and miR-363-3p[0.761(0.669～0.798)],and the differences were statistically significant (t=6.026,7.769,all P<0.05). Conclusion High levels of serum miR-424 expression and low levels of miR-363-3p expression suggested MM progression and poor prognosis. The combination of serum miR-424 and miR-363-3p has a high value in predicting the progression and prognosis of MM.
